We narrowed to 10,319 results for: 158

1. pGFP-HBO1-G485A pc2201

   Plasmid

   #167570

   Purpose

   The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

   Depositor

   Cristina Cardoso

   Article

   Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

   Insert

   histone acetyltransferase 1 (KAT7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G485A

   Promoter

   CMV

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_Anc689-NG-nCas9

   Plasmid

   #163527

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a-His6-LOXCATmut

   Plasmid

   #140085

   Purpose

   A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-anti-PDL1(KN035) PAGER(TF)

   Plasmid

   #230003

   Purpose

   Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2671

   Plasmid

   #142972

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NEUROD4 (NEUROD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT/CamKII gamma CA

   Plasmid

   #122142

   Purpose

   For making constitutively active CamKII gamma RNA

   Depositor

   Carmen Williams

   Article

   Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   constitutively active CamKII gamma (Camk2g Mouse)
   

   Use

   For in vitro transcription

   Tags

   Expression

   Mutation

   First 291 amino acid residues of CaMKII gamma

   Promoter

   T7

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

   Plasmid

   #175563

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL1
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZ403

   Plasmid

   #208113

   Purpose

   mSwAP-In marker cassette 1 with mScarlet-PuroTK

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mScarlet-PuroTK
   

   Use

   Tags

   P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163533

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30-ARH3-His-Sumo-HA

    Plasmid

    #111578

    Purpose

    Expresses ARH3 (amino acids 14-363) in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    ARH3 (ADPRS Human)
    

    Use

    Tags

    6xHis-Sumo and HA

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-13

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX4T2 RANK-L

    Plasmid

    #108571

    Purpose

    Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

    Depositor

    Jean-Claude Scimeca

    Article

    Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

    Insert

    RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa158-aa316

    Promoter

    Ptac (trp/lac)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Intersectin Short

    Plasmid

    #47394

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    Intersectin short (ITSN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Flag-hNF2

    Plasmid

    #107150

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0748

    Plasmid

    #141683

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PIAS4 (PIAS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-His:CRY2:nls:VPR

    Plasmid

    #135989

    Purpose

    Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Inserts

    CRY2 domain fused to the VPR activation domain
    CRY2 domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZ854

    Plasmid

    #208116

    Purpose

    mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mNeonGreen-HPRT1-Neo
    

    Use

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZ852

    Plasmid

    #208114

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-HygTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-HygTK
    

    Use

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DPYD-FLAG

    Plasmid

    #61616

    Purpose

    The human DPYD was used to study the effect of DPYD expression on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (DPYD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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