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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5/FRT/TO V5 DNAJB6b

Plasmid

#19528

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6b (DNAJB6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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3543 pMIG Bcl-XL mut8

Plasmid

#8792

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G138A, R139L, I140N

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-His:PYL1:nls:VPR

Plasmid

#135988

Purpose

Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

PYL1 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-595

Plasmid

#11623

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GNPTAB

Plasmid

#78108

Purpose

Mammalian expression of human GNPTAB with C terminal myc tag

Depositor

Thomas Braulke

Article

Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

Insert

GNPTAB (GNPTAB Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
N-Myc-53BP1 D1521A pLPC-Puro

Plasmid

#19837

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Mutation

changed Asp1521 to Ala

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

Plasmid

#191164

Purpose

Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (additional cutsites)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-BR1

Plasmid

#160333

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

Insert

BR1

Tags

6xHis tag

Expression

Bacterial

Promoter

T5

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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PpyCAG-NANOG-IB

Plasmid

#60440

Purpose

Transient human NANOG expression

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

NANOG (NANOG Human)

Expression

Mammalian

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBItetDT12nGFP

Plasmid

#80420

Purpose

tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

Depositor

Thomas Cooper

Article

Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct6

Plasmid

#28199

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-314 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct2

Plasmid

#28195

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa86-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191156

Purpose

Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct7

Plasmid

#28200

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-274 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pBabe Angptl4 puro

Plasmid

#19156

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

angiopoietin-like 4 (ANGPTL4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only