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pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TC9pAB

Plasmid

#71310

Purpose

used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

Depositor

Karl Degenhardt

Insert

Cas9

Use

In vitro transcription

Tags

NLS

Promoter

T7

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RRR

Plasmid

#48636

Purpose

Expression of TALEN proteins with FokI-RRR domain

Depositor

David Grunwald

Publication

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

HA-1 and HA-2

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Neo Myr Flag RPS6KA2

Plasmid

#20621

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZL Neo Myr Flag CAMK2B

Plasmid

#20439

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMK2B (CAMK2B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2TAL3-DDD

Plasmid

#48637

Purpose

Expression of TALEN proteins with FokI-DDD domain

Depositor

David Grunwald

Publication

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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L444P GBA pcDNA3.1

Plasmid

#188582

Purpose

Ectopic expression L444P GBA

Depositor

Anthony Schapira

Publication

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

L444P

Promoter

CMV

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-T2A-moxMaple3

Plasmid

#120875

Purpose

Mammalian expression of mCherry and moxMaple3

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

mCherry
moxMaple3

Expression

Mammalian

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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U6-BbsI-GGsite-mCherry-Puro

Plasmid

#221232

Purpose

Cloning vector for sgRNA with mCherry and Puro

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Type

Empty backbone

Expression

Mammalian

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCB24 pDEST crybb1:TagBFP

Plasmid

#195987

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Publication

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHD066

Plasmid

#84032

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Publication

Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi:

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and Elastase

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBT137-splitC

Plasmid

#122605

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtYAO

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCORE

Plasmid

#72231

Purpose

Contains the CORE cassette with KlURA3 and KanMX4 markers

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Nat Biotechnol. 2001 Aug;19(8):773-6.

Inserts

KlURA3
kanMX4

Expression

Bacterial

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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