Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 278 results for: plasmid transfection

Clear keywords
Showing: 41 - 80 of 278 results

  19. pcDNA3.1-ME-Flag

    Plasmid
    #101860
    Purpose
    Mds1-Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101860-plasmid-map-sequence-id-211085

    Enlarge

  20. pCSThaP6ERT(#184)

    Plasmid
    #184103
    Purpose
    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    HA-MmPax6-ERT2
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6+T7
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184103-plasmid-map-sequence-id-365942

    Enlarge

  21. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184064-plasmid-map-sequence-id-365948

    Enlarge

  22. pCSTG4FER3(#190)

    Plasmid
    #184069
    Purpose
    cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184069-plasmid-map-sequence-id-365692

    Enlarge

  23. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230055-plasmid-map-sequence-id-457173

    Enlarge

  24. pCSmp38ERT(#143)

    Plasmid
    #184077
    Purpose
    cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfP38a-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184077-plasmid-map-sequence-id-365706

    Enlarge

  26. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid
    #223505
    Purpose
    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
    Tags
    SNAP-tag
    Expression
    Mammalian
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223505-plasmid-map-sequence-id-443299

    Enlarge

  27. pCSZPKIamutHAERT(#119)

    Plasmid
    #184076
    Purpose
    cyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfmutPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…
    Promoter
    sCMV+SP6
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184076-plasmid-map-sequence-id-365868

    Enlarge

  28. pCSZPKIaHAERT(#118)

    Plasmid
    #184075
    Purpose
    cyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184075-plasmid-map-sequence-id-365717

    Enlarge

  29. pCSm5En2DER4(#1062)

    Plasmid
    #184093
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>D)-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    chkEn2: C175D; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184093-plasmid-map-sequence-id-365715

    Enlarge

  30. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Human, Homo sapiens)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human UbC
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115239-plasmid-map-sequence-id-222083

    Enlarge

  31. pAAV2-CAGIG-LAMP1-mGL

    Plasmid
    #222394
    Purpose
    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
    Depositor
    Pantelis Tsoulfas
    Insert
    LAMP1 and mGreenlantern (LAMP1 Synthetic, Human, Homo Sapiens)
    Use
    AAV
    Tags
    LAMP1 and mGreenLantern
    Expression
    Mammalian
    Promoter
    CAG or CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222394-plasmid-map-sequence-id-446519

    Enlarge

  32. pCAG-GluA1-scFV-Halo

    Plasmid
    #230065
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230065-plasmid-map-sequence-id-460116

    Enlarge

  33. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

    Enlarge

Showing: 41 - 80 of 278 results