Skip to main content

More Filters

We narrowed to 172 results for: LPP

Showing: 51 - 100 of 172 results
  1. pKB252

    Plasmid
    #170035
    Purpose
    Encodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    K6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pKB254

    Plasmid
    #170037
    Purpose
    Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    H6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  3. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Insert
    αNTD
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pKB231

    Plasmid
    #170018
    Purpose
    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Cecropin P1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pKB236

    Plasmid
    #170027
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    TCP
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 31, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pKB232

    Plasmid
    #170019
    Purpose
    Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CRAMP
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pKB222

    Plasmid
    #170012
    Purpose
    Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    LL-37
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pKB230

    Plasmid
    #170017
    Purpose
    Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Protegrin-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pKB226

    Plasmid
    #170016
    Purpose
    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Defensin HNP-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pKB255

    Plasmid
    #170038
    Purpose
    Encodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Melittin
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Jan. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pKB235.2

    Plasmid
    #170025
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL20 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pKB237.2

    Plasmid
    #170032
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL19 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pKB234

    Plasmid
    #170021
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    hBD3
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  17. pKB234.1

    Plasmid
    #170022
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    hBD3 alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  18. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Insert
    alpha NTD-Beta flap fusion
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  19. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pKB239

    Plasmid
    #170034
    Purpose
    Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Granulysin (GNLY Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pKB234.2

    Plasmid
    #170023
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    hBD3 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Dec. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. VN515 pBR-rpoA-IP1

    Plasmid
    #235098
    Purpose
    Quantitative bacterial two-hybrid (qB2H). Expresses IP1.
    Depositor
    Insert
    IP1
    Use
    Synthetic Biology; Quantitative bacterial two-hyb…
    Tags
    rpoA
    Expression
    Bacterial
    Promoter
    lpp+lacUV5
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  24. VN516 pBR-rpoA-IP2

    Plasmid
    #235099
    Purpose
    Quantitative bacterial two-hybrid (qB2H). Expresses IP2.
    Depositor
    Insert
    IP2
    Use
    Synthetic Biology; Quantitative bacterial two-hyb…
    Tags
    rpoA
    Expression
    Bacterial
    Promoter
    lpp+lacUV5
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. VN517 pBR-rpoA-IP3

    Plasmid
    #235100
    Purpose
    Quantitative bacterial two-hybrid (qB2H). Expresses IP3.
    Depositor
    Insert
    IP3
    Use
    Synthetic Biology; Quantitative bacterial two-hyb…
    Tags
    rpoA
    Expression
    Bacterial
    Promoter
    lpp+lacUV5
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. VN518 pBR-rpoA-IP4

    Plasmid
    #235101
    Purpose
    Quantitative bacterial two-hybrid (qB2H). Expresses IP4.
    Depositor
    Insert
    IP4
    Use
    Synthetic Biology; Quantitative bacterial two-hyb…
    Tags
    rpoA
    Expression
    Bacterial
    Promoter
    lpp+lacUV5
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pKB237.1

    Plasmid
    #170031
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL19 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  28. pKB235.4

    Plasmid
    #170026
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL20 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  29. pKB236.1

    Plasmid
    #170028
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    TCP Δ C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  30. pKB236.2

    Plasmid
    #170029
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    TCP C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Availability
    Academic Institutions and Nonprofits only
  31. pKB237

    Plasmid
    #170030
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL19 (CCL19 Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  32. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available Since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
  33. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  34. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  35. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL20 (CCL20 Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  36. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  37. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  38. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  39. pDule2-para-aminoPhe

    Plasmid
    #85503
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  40. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 172 results