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Plasmid#185589Purposeto see protein dynamicsDepositorInsertVasa-S636K
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationS636KPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8NmCherry-ABCB4a
Plasmid#34941DepositorInsertABCB4a
UseSea urchin, zebrafish, xenopusTagsmCherryExpressionMammalianMutationPromoterSP6Available sinceJan. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8NCerulean-ABCB4a
Plasmid#34945DepositorInsertABCB4a
UseSea urchin, zebrafish, xenopusTagsCeruleanExpressionMammalianMutationPromoterSP6Available sinceJan. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8CCerulean-ABCG2a
Plasmid#34946DepositorInsertABCG2a
UseSea urchin, zebrafish, xenopusTagsCeruleanExpressionMammalianMutationPromoterSP6Available sinceJan. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+NmCitrine-ABCB1a
Plasmid#34956DepositorInsertABCB1a
UseSea urchin, zebrafish, xenopusTagsmCitrineExpressionMammalianMutationPromoterSP6Available sinceJan. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB5225
Plasmid#215492PurposeA pUra4 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1DepositorInsertPil1-mCherry
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB5253
Plasmid#215501PurposeA pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1DepositorInsertmECitrine-CtNbr1
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MiniSOG-SpAGS-GFP
Plasmid#185581Purposefor CALIDepositorInsertminiSOG-SpAGS
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpAGS-dGoLoco1-GFP
Plasmid#185577Purposefor overexpressionDepositorInsertAGS-dGoLoco1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationdeletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-SpBeta-catenin
Plasmid#185579Purposeto see protein dynamicsDepositorInsertBeta-catenin
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0327
Plasmid#133488PurposeExpression of nucleo-cytoplasmic sfGFPDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0471
Plasmid#133490PurposeExpression of nucleo-cytoplasmic mTagBFP2DepositorInsertpUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0624
Plasmid#133491PurposeExpression of cytoplasmic 3mTagBFP2DepositorInsertpUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
NmCherry-SpVasa
Plasmid#66786Purposeexpression of mCherry in Sea Urchin germ cellsDepositorInsertVasa
UseSea urchin, zebrafish, xenopusTagsmCherryExpressionMammalianMutationPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xenopus twisted gastrulation
Plasmid#24974DepositorInserttwisted gastrulation (Twsg1 Mouse)
UseTagsFLAG and chordin signal peptideExpressionMammalianMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa-C3
Plasmid#185587Purposeto recruit Vasa-C3 to membraneDepositorInsertVasa-C3
UseIn vitro transcription (mrna synthesis)TagsmCherry and membrane targeting sequence from Neur…ExpressionMutationC3 mutantPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa
Plasmid#185586Purposeto recruit Vasa to membraneDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsmCherry, S-Tag and membrane targeting sequence fr…ExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0785
Plasmid#133507PurposeExpression of mCherry tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0752
Plasmid#133486PurposeExpression in fission yeast from promoter pnmt41 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0753
Plasmid#133487PurposeExpression in fission yeast from promoter pnmt81 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 mouse CV2 PC
Plasmid#25776DepositorInsertcross veinless 2 (Bmper Mouse)
UseTagsPCExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAV0749
Plasmid#133483PurposeExpression in fission yeast from promoter ptdh1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0714
Plasmid#133479PurposeExpression in fission yeast from promoter pact1, integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0750
Plasmid#133484PurposeExpression in fission yeast from promoter purg1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0835
Plasmid#133512PurposeExpression of mCherry tagged CHD domain to monitor actin cytoskeletonDepositorInsertpUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0747
Plasmid#133481PurposeExpression in fission yeast from promoter ppom1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0748
Plasmid#133482PurposeExpression in fission yeast from promoter ppak1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0605
Plasmid#133503PurposeExpression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0746
Plasmid#133480PurposeExpression in fission yeast from promoter prga3 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-SFFV.Reporter.RFP657.PAC
Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Plagl2
Plasmid#228061Purposetransiently express mouse Plagl2DepositorInsertPlagl2 (Plagl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.PAC
Plasmid#57825PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter drivenDepositorInsertssgRNA
EFS
PAC
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLViP-LifeAct-iRFP670
Plasmid#229699PurposeLentiviral expression of LifeAct-iRFP670 in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.tRFP
Plasmid#57826PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLViP-LifeAct-TagRFPT
Plasmid#229700PurposeLentiviral expression of LifeAct-TagRFPT in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsTagRFPTExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-Ccnd2PromoterRegion1(1.2kb)
Plasmid#228058Purpose1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporterDepositorInsertCcnd2 promoter (Ccnd2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-Ccnd2PromoterRegion2(0.5kb)
Plasmid#228059Purpose0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporterDepositorInsertCcnd2 promoter (Ccnd2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-Igf2Promoter
Plasmid#228060Purpose0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporterDepositorInsertIgf2 promoter (Igf2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
aL-EGFP
Plasmid#103879Purposefull-length human aL with EGFP at betapropellerDepositorInsertfull-length human aL with EGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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aL-L3
Plasmid#103881Purposefull-length human aL with moxsynGFP in betapropellerDepositorInsertfull-length human aL with moxsynGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only