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pTwist-msEEA1

Plasmid

#214991

Purpose

Gateway entry vector with mouse EEA1

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

Gateway

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214997

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (Tmem115 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214996

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (TMEM115 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218633

Purpose

Proximity biotinylation of msNLRP3

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g1 lentiCRISPRv2-mCherry

Plasmid

#218652

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

Plasmid

#214998

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

Plasmid

#214999

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE pGK hNLRP3-mNG

Plasmid

#218643

Purpose

Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

pGK

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3-mNG-STOP

Plasmid

#218644

Purpose

Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

Expression

Mutation

R262W

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

Plasmid

#214989

Purpose

Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

CRISPR

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

Plasmid

#214990

Purpose

Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

CRISPR

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

Plasmid

#214994

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

Plasmid

#210604

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti UBC hNLRP3-mNG

Plasmid

#218642

Purpose

Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Tags

Expression

Mutation

Promoter

CAG

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry

Plasmid

#210603

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

Plasmid

#210605

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional base editor

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

ABE8e-hyperdCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GOLIM4 g2 LentiCRISPRv2-mCherry

Plasmid

#218663

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218634

Purpose

Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

delPYD (I125M-END)

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GOLIM4 g1 LentiCRISPRv2-mCherry

Plasmid

#218662

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-mApple-6xHp

Plasmid

#217376

Purpose

Lipid droplet localization of 6xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

mApple-6x Hp NC

Use

Lentiviral

Tags

mApple

Expression

Mutation

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Use

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Use

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-mApple-6xHp

Plasmid

#214412

Purpose

Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

mApple-6xHp

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-mApple-mito

Plasmid

#214415

Purpose

Mitochondria localization of low-affinity NFAST fused to mApple-mito

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

mApple-mito

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-pAG-Hia5

Plasmid

#174373

Purpose

Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein AG - hia5

Use

Tags

6xHis and Protein A/G

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-pA-Hia5

Plasmid

#174372

Purpose

SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein A - hia5

Use

Tags

6xHis and Protein A

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-6xHp

Plasmid

#214410

Purpose

Lipid droplet localization of 6xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Use

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Use

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-mito)

Plasmid

#214423

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito

Use

Lentiviral

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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