1. Mouse c-myc (exon I)

   Plasmid

   #8446

   Depositor

   Frederic Mushinski

   Article

   Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

   Insert

   Mouse c-myc (exon I) (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pVITRO1-BFP-NES

   Plasmid

   #184461

   Purpose

   Cytosolic EBFP2 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   EBFP2-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTL385

   Plasmid

   #168446

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   OsaHIP39
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

   Plasmid

   #148446

   Purpose

   Mammalian Expression of 6xbsMS2-PolyA-Malat

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   6xbsMS2-PolyA-Malat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUAST td tomato Rab5 WT

   Plasmid

   #58446

   Purpose

   expression of drosophila td tomato Rab5

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   td tomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA4/TO-mu24 1-216-2xStrep

   Plasmid

   #138446

   Purpose

   Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   mu24
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Amino acids 1-216

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUW-TetO-NMI

   Plasmid

   #178446

   Purpose

   doxycycline-inducible expression of Human NMI in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   NMI (NMI Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

   Plasmid

   #184463

   Purpose

   pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.

   Depositor

   Rhonda Kineman

   Article

   Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757.

   Insert

   N-Flag-mSTAT5bCA (Stat5b Mouse)
   

   Use

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   Point mutation at base 1963 (A to C), which chang…

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

   Plasmid

   #184464

   Purpose

   Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gZiPro W83F

   Plasmid

   #198446

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W83F

   Promoter

   T7 promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-ZNF658

    Plasmid

    #88446

    Purpose

    Donor Vector containing ZNF658 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF658 (ZNF658 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Availability

    Academic Institutions and Nonprofits only

11. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RCH1:HDT1,HDT2-RNAi

    Plasmid

    #108446

    Purpose

    Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

    Depositor

    Ton Bisseling

    Article

    Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

    Inserts

    HDT1 (HDA3 Mustard Weed)
    HDT2 (HD2B Mustard Weed)
    

    Use

    RNAi

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RCH1

    Availability

    Academic Institutions and Nonprofits only

13. pUltra-MDH1-ME1

    Plasmid

    #184465

    Purpose

    Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydrogenase 1
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Lentiviral

    Tags

    fused to T2A

    Expression

    Mammalian

    Mutation

    deletion of stop codon

    Promoter

    UbC

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SLX4

    Plasmid

    #73242

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SLX4 (SLX4 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSB700-mouse-ACTC1-Puro

    Plasmid

    #128326

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUPD2-GFP control (B3-B5)

    Plasmid

    #211848

    Purpose

    GFP CDS in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUPD2-N-L-mCherry2 (B5)

    Plasmid

    #211849

    Purpose

    CDS for mCherry in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUPD2-HR1b-C (B3-B4)

    Plasmid

    #211851

    Purpose

    CDS for HR1b degron sequence in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2-N-L-HR1b (B4-B5)

    Plasmid

    #211852

    Purpose

    CDS for HR1b degron sequence in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD2-HR1b-GFP-L (B3-B4)

    Plasmid

    #211853

    Purpose

    E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge