We narrowed to 96 results for: Neisseria

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pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

Plasmid

#85750

Purpose

Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type II-C anti-CRISPR AcrIIC3Nme
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKS7107

Plasmid

#89051

Purpose

Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Nm crRNA
Nm tracrRNA
hNmCas9

Use

CRISPR

Tags

HA tag, NLS, and SV40 NLS

Promoter

EF1a and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-SPMc-SpyCatcher003-Ctag

Plasmid

#246648

Purpose

Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

His6-SUMO-SPMc-SpyCatcher003-Ctag

Tags

Ctag, His-tag, SUMO, and SpyCatcher003

Expression

Bacterial

Mutation

The SPMc comprises the C-terminal fragment of th…

Promoter

T7

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mCherry

Plasmid

#196664

Purpose

Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Tags

TEV-cleavable distrep tag II and mCherry

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003

Plasmid

#246647

Purpose

Expresses AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 in bacterial cells for calcium-induced NeissLock protein ligation

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003

Tags

AviTag, His-tag, and SpyTag003

Expression

Bacterial

Mutation

Orinithine Decarboxylase Antizyme (OAZ) with C175…

Promoter

T7

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-MtrR

Plasmid

#51188

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

MtrR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149S/F366A

Plasmid

#100165

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149S, F366A

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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NcCas9

Plasmid

#194093

Purpose

Expresses human codon optimized NcCas9. Genome editing in human cells.

Depositor

Zhanjun Li

Article

Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

Insert

NcCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2698-CMV-Nme2Cas9-D56R

Plasmid

#228719

Purpose

Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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nNc-ABE

Plasmid

#194095

Purpose

CMV-TadA 8e-nNcCas9. A-to-G base editing in human cells.

Depositor

Zhanjun Li

Article

Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

Insert

TadA 8e-nNcCas9

Expression

Mammalian

Mutation

NcCas9 D16A

Promoter

CMV

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSimpleII-NmCas9-FLAG

Plasmid

#47872

Purpose

FLAG tagged NmCas9 with no NLS.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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