We narrowed to 82 results for: Neisseria

1. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

   Plasmid

   #85750

   Purpose

   Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type II-C anti-CRISPR AcrIIC3Nme
   mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NlaCascade-NLS-6xHis

   Plasmid

   #180213

   Purpose

   Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas5-Cas8-Cas7
   

   Use

   Tags

   6XHis and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPglL-strep-mCherry

   Plasmid

   #196664

   Purpose

   Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   PglL
   

   Use

   Tags

   TEV-cleavable distrep tag II and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPglL-strep-mClover3

   Plasmid

   #196665

   Purpose

   Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   PglL
   

   Use

   Tags

   TEV-cleavable di-strep tag II and mClover3

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET26-CNTnw

   Plasmid

   #100163

   Purpose

   recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-MtrR

   Plasmid

   #51188

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   MtrR
   

   Use

   Synthetic Biology

   Tags

   6x-his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET26-CNTN149L

   Plasmid

   #100164

   Purpose

   recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   N149L

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET26-CNTN149S/F366A

   Plasmid

   #100165

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   N149S, F366A

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. NcCas9

    Plasmid

    #194093

    Purpose

    Expresses human codon optimized NcCas9. Genome editing in human cells.

    Depositor

    Zhanjun Li

    Article

    Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

    Insert

    NcCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2699-CMV-Nme2Cas9-E932R

    Plasmid

    #228720

    Purpose

    Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-E932R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid

    #228717

    Purpose

    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-D56R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEJS2698-CMV-Nme2Cas9-D56R

    Plasmid

    #228719

    Purpose

    Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-D56R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. nNc-ABE

    Plasmid

    #194095

    Purpose

    CMV-TadA 8e-nNcCas9. A-to-G base editing in human cells.

    Depositor

    Zhanjun Li

    Article

    Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

    Insert

    TadA 8e-nNcCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NcCas9 D16A

    Promoter

    CMV

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ839

    Plasmid

    #39317

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (NMA0631 Neisseria meningitidis)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pSimpleII-NmCas9-FLAG

    Plasmid

    #47872

    Purpose

    FLAG tagged NmCas9 with no NLS.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. NmMetNI-WT

    Plasmid

    #172114

    Purpose

    Expression of MetN and MetI membrane proteins

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Inserts

    MetN
    MetI
    

    Use

    Tags

    10x His Tag and Enterokinase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

    Plasmid

    #228727

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

    Plasmid

    #228728

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge