We narrowed to 58,151 results for: SAP

1. pR-mT-Sapphire

   Plasmid

   #228597

   Purpose

   Mammalian expression of mT-Sapphire, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mT-Sapphire
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM-mT-Sapphire

   Plasmid

   #228616

   Purpose

   Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mT-Sapphire
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPt_Sap

   Plasmid

   #206856

   Purpose

   A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.

   Depositor

   Bogumil Karas

   Article

   Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH-Sapphire

   Plasmid

   #176566

   Purpose

   Expression of Sapphire for auxotrophic selection in the absence of lysine

   Depositor

   Markus Ralser

   Insert

   yETSapphire
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU-Sapphire

   Plasmid

   #176567

   Purpose

   Expression of Sapphire for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Insert

   yETSapphire
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM-Sapphire

   Plasmid

   #176569

   Purpose

   Expression of Sapphire for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Insert

   yETSapphire
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAHISMRSAPPFK

   Plasmid

   #97003

   Purpose

   Expressing MRSA PPFK protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Putative phosphofructokinase
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_SAP130_2

   Plasmid

   #86311

   Purpose

   Encodes gRNA for 3' target of human SAP130

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against SAP130 (SAP130 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBsaP450camGG

   Plasmid

   #85101

   Purpose

   P450cam (CYP101A1)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450cam
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBsaPro5X

    Plasmid

    #85100

    Purpose

    Putidaredoxin fused to Pro5

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-ASAP3ER

    Plasmid

    #195356

    Purpose

    ASAP3ER under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sapphireCKAR(T/A)

    Plasmid

    #118469

    Purpose

    Negative-control mutant for sapphireCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-SapIR

    Plasmid

    #165537

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    SapI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCAG-postASAP

    Plasmid

    #178792

    Purpose

    Expresses voltage sensor targeted to dendrites and spines

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95 and ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCP20T-tsapphire

    Plasmid

    #78464

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    T-Sapphire
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPICZalphaB-SapL3

    Plasmid

    #78171

    Purpose

    Expression of Saporin L3 in pichia

    Depositor

    Vern Schramm

    Article

    Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

    Insert

    Saporin L3
    

    Use

    Tags

    alpha factor

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sirius-mT-Sapphire_pcDNA3

    Plasmid

    #89584

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    Sirius-mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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