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We narrowed to 6,501 results for: nls

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Showing: 21 - 40 of 6501 results

  2. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Publication
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Promoter
    p10 promoter
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    13763-plasmid-map-sequence-id-364424

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  11. pCAG-T3-hCAS-pA

    Plasmid
    #48625
    Purpose
    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii, Kunihiko Naito
    Publication
    Fujii et al Nucleic Acids Res. 2013 Aug 30.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    FLAG, NLS, and polyA
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    48625-plasmid-map-sequence-id-116741

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  12. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    72247-plasmid-map-sequence-id-485302

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  14. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    50588-plasmid-map-sequence-id-75660

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  15. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid
    #64222
    Purpose
    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    sgRNA cassette
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    64222-plasmid-map-sequence-id-107958

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  17. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    65771-plasmid-map-sequence-id-112653

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  19. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    50581-plasmid-map-sequence-id-520531

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  20. pICE-NLS-mCherry

    Plasmid
    #60364
    Purpose
    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-mCherry
    Tags
    NLS from SV40 T antigen and mCherry
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    60364-plasmid-map-sequence-id-96649

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Showing: 21 - 40 of 6501 results