We narrowed to 6,172 results for: nls

1. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

   Plasmid

   #191368

   Purpose

   Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dHgm4Cas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

   Plasmid

   #69233

   Purpose

   R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

   Plasmid

   #69231

   Purpose

   Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 TALE
   

   Use

   CRISPR

   Tags

   2X NLS and NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. His_CL7_2NLS_iCas12a-4NLS

   Plasmid

   #222971

   Purpose

   Bacterial expression of NLS-rich iCas12a construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His_CL7_2NLS_iCas12a-4NLS
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

   Plasmid

   #222989

   Purpose

   Bacterial expression of iGeoCas9(G) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(G)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

   Plasmid

   #222988

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

   Plasmid

   #222987

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

   Plasmid

   #107300

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP
   

   Use

   CRISPR

   Tags

   3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUB-nSypHer (NLS:sypHer:NLS)

    Plasmid

    #84731

    Purpose

    Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    nSypHer
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

    Plasmid

    #244836

    Purpose

    Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

    Plasmid

    #244839

    Purpose

    Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. L_NLS-mutNLS V5-Twist

    Plasmid

    #201381

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A4-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. M_NLS-mutNLS V5-Twist

    Plasmid

    #201379

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A5-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H_NLS-mutNLS V5-Twist

    Plasmid

    #201378

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. UL_NLS-mutNLS V5-Twist

    Plasmid

    #201382

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A3-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSimpleII-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47867

    Purpose

    This plasmid encode NmCas9 with two NLS and a HA tag

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_NLS-dSaCas9-NLS-VPR

    Plasmid

    #68495

    Purpose

    AAV vector containing nuclease null SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    dSaCas9
    

    Use

    AAV

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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