Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 1,254 results for: sumo

Showing: 21 - 40 of 1254 results

  2. SUMO-PNGaseF

    Plasmid
    #228498
    Purpose
    10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    ngl
    Use
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228498-plasmid-map-sequence-id-455750

    Enlarge

  3. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Use
    Tags
    His8-Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

    Enlarge

  5. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Use
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Use
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available Since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

    Enlarge

  17. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

    Enlarge

  19. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Use
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available Since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

    Enlarge

Showing: 21 - 40 of 1254 results