Addgene

We narrowed to 1,846 results for: Ipp

Showing: 571 - 600 of 1846 results

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MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Use

Tags

Expression

Mammalian

Mutation

Crippled Kozac sequence

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBT255_(pCA-GFP4m)

Plasmid

#36877

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

F64L; S65T; V163A; S175G

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-GFP

Plasmid

#209699

Purpose

Expresses GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

CBA

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEF mito-PAcGFP

Plasmid

#188905

Purpose

Expresses mitochondrial photoactivatable GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial photoactivatable GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-TARG1

Plasmid

#172577

Purpose

For gateway cloning of C-terminal tagged TARG1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pZF-AA-GCN

Plasmid

#127479

Purpose

coding sequence for synthetic zinc finger ZF-AA-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AA fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Expression

Mutation

Promoter

T7

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPAmCherry3-C1

Plasmid

#31933

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry3

Use

Tags

Expression

Mammalian

Mutation

M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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PCDNA3-GFP1-9 T2A mCherry

Plasmid

#124430

Purpose

Expresses GFP1-9 and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9 T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcDNA3

Plasmid

#18964

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and linked by IRES

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

Promoter

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pDV10_pCMV-ADAR1-p150

Plasmid

#200405

Purpose

Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p150

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDV09_pCMV-ADAR1-p110

Plasmid

#200404

Purpose

Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p110

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pPAmCherry2-C1

Plasmid

#31932

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry2

Use

Tags

Expression

Mammalian

Mutation

M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBT346_pLambda-attB-pCA-GFP-pA

Plasmid

#52551

Purpose

for producing PhiC31 attB-containing minicircle in vitro

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

Lambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120392

Purpose

pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

U6

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Article

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200406

Purpose

Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

MCP-ADAR2dd(E488Q)-NES

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

E488Q (hyperactive)

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-cyto-GCaMP6f

Plasmid

#52925

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Article

Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

Has Service

AAV5

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

U6 and CBh

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Clomeleon

Plasmid

#203723

Purpose

A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

Depositor

George Augustine

Article

Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

Insert

cyan fluorescent protein linked yellow florescent protein

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Cyan Fluorescent Protein, Topaz Fluorescent Prote…

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pPAmCherry-b-actin

Plasmid

#31949

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

b-actin

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pWPT-PDX1

Plasmid

#12256

Depositor

Didier Trono

Article

Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

Insert

Pdx1 (PDX1 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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