We narrowed to 7,580 results for: Ski;
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Plasmid#20223DepositorInsert3xFRB (MTOR Human)
TagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneExpressionBacterialPromotergapDH promoter from Eggerthella lentaAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 POLD1
Plasmid#67128PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G02
Plasmid#67136PurposeGateway destination vector for cell-free expression with C-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G04
Plasmid#67138PurposeGateway destination vector for cell-free expression with C-term eGFP 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HISAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AKAP1-mCherry-LOVPLD
Plasmid#219558Purposemitochondria-targeted LOVPLDDepositorInsertAKAP1-mCherry-LOVPLD
TagsmCherryExpressionMammalianAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optodPLD
Plasmid#140055PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
Tags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationH170A (catalytic dead)Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP2B
Plasmid#67092PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
Plasmid#173453PurposeContains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g1 Cas9-T2A-mCherry
Plasmid#164250PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgTREX1
UseCRISPRExpressionMammalianPromoterU6Available SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RAD54B
Plasmid#67124PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC-PTP-NEO
Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)Available SinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA
Plasmid#164795PurposepLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.DepositorInsertGluN2B
UseLentiviralTags4xMycAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pN-PURO-PTP
Plasmid#51711Purposecontains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)Available SinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyExpressionBacterialPromoterJ23100 constitutiveAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-2xFRB
Plasmid#20224DepositorInsert2xFRB (MTOR Human)
TagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyExpressionBacterialPromoterPTACAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP204-Lenti-TRE3G-GFP-WPRE-Pa
Plasmid#164788PurposepLenti vector backbone designed to express GFP from a TRE3G promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralPromoter1.3αCaMKIIAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP
Plasmid#67743PurposePr promoter expressing deGFPDepositorInsertUTR1-deGFP-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-PrAvailable SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G10
Plasmid#67144PurposeGateway destination vector for cell-free expression with C-term sfGFP 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHIS and sfGFPAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SP2
Plasmid#67109PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 USF2
Plasmid#67069PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 VPS29
Plasmid#67052PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC zeta
Plasmid#8425DepositorInsertMouse Protein Kinase C zeta (Prkcz Mouse)
ExpressionBacterialAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA
Plasmid#62931PurposepLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).DepositorTypeEmpty backboneUseLentiviralPromoter1.3αCaMKIIAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K410N
Plasmid#87280PurposeEncodes Hsh155 K410N for expression in yeastDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFX1
Plasmid#67131PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G05
Plasmid#67139PurposeGateway destination vector for cell-free expression with N-term 8xHis mCherry tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis and mCherryAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 S100A1
Plasmid#67048PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06
Plasmid#67140PurposeGateway destination vector for cell-free expression with C-term mCherry 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHIS and mCherryAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJEP216-Lenti-1.3CaMKIIa P-GFP-WPRE-pA
Plasmid#62926PurposepLenti vector backbone designed to express GFP from a 1.3CaMKIIa promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralPromoter1.3αCaMKIIAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G09
Plasmid#67143PurposeGateway destination vector for cell-free expression with N-term 8xHis sfGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis and sfGFPAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SREBF2
Plasmid#67129PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only