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pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCL6.1.0-gDNA
Plasmid#112485PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6DepositorInsertBCL6 (BCL6 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15
Plasmid#112761Purposeexpress GFP-4EBP1-Rheb15DepositorInsertFlag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
UseLentiviralTagsFlag eGFP and Rheb15ExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmTurquoise
Plasmid#112961PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertITPKA
UseTags3x mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo
Plasmid#112684PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinaseDepositorInsertiRFP713
UseAAVTags2A-Flpo and Nuclear localization signalExpressionMutationPromoterCMV-IEAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIGH
Plasmid#112804Purposegateway cloning destination vector to express enhancer driven Gal4 in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF.1.0-gDNA
Plasmid#112395PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERFDepositorInsertERF (ERF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptdTHC
Plasmid#112617Purposepromoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2DepositorInsertsUsePromoterless vector, ready for promoter for mamma…TagstdTomatoExpressionMammalianMutationPromoterNo PromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-BFP HDRT Source (pTR 145)
Plasmid#112014PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFPDepositorInsertRAB11A-BFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1
Plasmid#112646PurposeExpression S.cerevisiaeDepositorInsertGcs1
UseTagsExpressionYeastMutationPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LPA2R-P2A-mCherry
Plasmid#112932PurposePlasmid co-producing the LPA2 receptor and a mCherryDepositorInsertLPA2 receptor
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-Bla
Plasmid#112561PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-Bla and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOP5MP
Plasmid#112612PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-MBP and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1665
Plasmid#112039PurposeIntegrating plasmid for expression of ER localized mCherry at TRP1 locusDepositorInsertER-mCherry
UseTagsExpressionYeastMutationPromoterTPIAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FRB-myc-RagA
Plasmid#112740Purposeexpress FRB-myc-RagADepositorInsertFRB-myc-RagA (RRAGA Human)
UseLentiviralTagsFRB, mycExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAT
Plasmid#112582PurposeUsed for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneUseTags6HisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
UseTags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only
