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Plasmid#163972PurposeHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pRS406 ATG1
Plasmid#166846PurposeExpresses Atg1 with ATG1 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS415-BUD32
Plasmid#10991DepositorInsertBUD32 (BUD32 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413-IVb
Plasmid#31459DepositorInsertlinker
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS303:3mCherry
Plasmid#37549DepositorInsert3mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 WT
Plasmid#87220PurposeEncodes Hsh155 WT for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS316-VTC4
Plasmid#108691PurposeVTC4 sequence expressed in yeast plasmidDepositorInsertVacuolar Transporter Chaperone 4
UseTagsExpressionYeastMutationPromoterVTC4 (native promoter)Available sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ACT_unstable
Plasmid#127608Purposehigh-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3DepositorInsertUnstable ACT1 intron (WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ECM_stable
Plasmid#127606Purposehigh-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3DepositorInsertStable ECM33 intron (WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
sub2-1 pRS315
Plasmid#111301Purposesub2-1 in pRS315DepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-1PromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#89966Purposecomplements prp8 genomic deletionDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#90964Purposeyeast vectorDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationPromoternativeAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415-NAR1
Plasmid#10995DepositorInsertNAR1 (NAR1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS304:3mCherry
Plasmid#37550DepositorInsert3mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pRS314-PRP16
Plasmid#89971PurposePrp16 in a trp backbone complements prp16 genomic deletionDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cwc25-pRSETA
Plasmid#61043PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.DepositorInsertCwc25
UseTagsTEV site, Histidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS316-Cwc25
Plasmid#111343PurposeCwc25 in pRS316(Ura) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only