Addgene

Showing: 381 - 400 of 5419 results

Results per page:

Sort results by:

ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGPD

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pADH2

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

Plasmid

#85039

Purpose

For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

RFP-P2A-tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

Plasmid

#195048

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

Plasmid

#195047

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

Plasmid

#195046

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM614-TRA-2::GFP

Plasmid

#27128

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 GFP (tra-2 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

replaced stop codon with AgeI and EcoRI added GFP…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Use

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
SCORE

Plasmid

#40232

Depositor

Wolfhard Almers

Article

An et al Science. 2004 Nov 5;306(5698):1042-6.

Insert

Snap25b (SNAP25 Human)

Use

Tags

CFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCUPI NPD-GFP

Plasmid

#1191

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 (SUP35 Budding Yeast)

Use

Cup1 promoter

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGRE NPD-GFP

Plasmid

#1197

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-2T-NM

Plasmid

#1127

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM (SUP35 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pJC25NM

Plasmid

#1126

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM stop (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only