We narrowed to 3,631 results for: WOL

1. pET-21a_2xNLS_LbCpf1_protein_expression

   Plasmid

   #114366

   Purpose

   Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

   Insert

   2xNLS_LbCpf1
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and hU6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-OTUB1 T134R-pcDNA3.1

   Plasmid

   #118211

   Purpose

   Expresses FLAG-OTUB1 T134R mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 134 to arginine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AsCas12a-DR-cr in PBCSK-

   Plasmid

   #126640

   Purpose

   Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyCas9 EMX1-nicking sgRNA

   Plasmid

   #169859

   Purpose

   SpyCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpyCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRCP

   Plasmid

   #195862

   Purpose

   Encodes chromogenic protein (CP). CP inducible with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Chromogenic Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-Flag-TTGZFP-FokI-DD

   Plasmid

   #18755

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   zinc finger nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   RNA expression vector for expressing the zinc fin…

   Promoter

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-ING1b

   Plasmid

   #79052

   Purpose

   expression of p33ING1b in mammalian cells

   Depositor

   Karl Riabowol

   Article

   Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

   Insert

   ING1 (p33 ING1Ab isoform) (ING1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. SauCas9 EMX1-nicking sgRNA

   Plasmid

   #169860

   Purpose

   SauCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCas12a-DR-cr in PBCSK-

    Plasmid

    #126641

    Purpose

    Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pegRNA_HEK4_20-7

    Plasmid

    #201974

    Purpose

    Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_20-7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

    Plasmid

    #107317

    Purpose

    Expresses SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9
    

    Use

    CRISPR

    Tags

    3xHA and 2xNLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

    Plasmid

    #120801

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    2xNLS_FnCpf1
    

    Use

    CRISPR

    Tags

    MBP and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69227

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FnoCas12a-DR-cr in PBCSK-

    Plasmid

    #126642

    Purpose

    Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SauCas9KKH CCR5-nicking sgRNA

    Plasmid

    #169863

    Purpose

    SauCas9KKH nicking sgRNA for CCR5

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9KKH CCR5-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69226

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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