Addgene

We narrowed to 3,611 results for: WOL

Showing: 381 - 400 of 3611 results

Results per page:

Sort results by:

pEW3: pET-32a Dot1L(2-416)

Plasmid

#124098

Purpose

Dot1L(2-416) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-416) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and hU6

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pB1H1-Zif268

Plasmid

#12612

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Zif268 in pB1H1 (Egr1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCas9 EMX1-nicking sgRNA

Plasmid

#169859

Purpose

SpyCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpyCas9 EMX1-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-ING1b

Plasmid

#79052

Purpose

expression of p33ING1b in mammalian cells

Depositor

Karl Riabowol

Article

Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

Insert

ING1 (p33 ING1Ab isoform) (ING1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_FnCpf1_2xNLS_protein_expression

Plasmid

#124548

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnCpf1_2xNLS

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Use

Tags

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
SauCas9 EMX1-nicking sgRNA

Plasmid

#169860

Purpose

SauCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-Flag-TTGZFP-FokI-DD

Plasmid

#18755

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Use

Tags

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pegRNA_HEK4_20-7

Plasmid

#201974

Purpose

Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_20-7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-DR-cr in PBCSK-

Plasmid

#126641

Purpose

Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO1-puro-U6-sgRNA-TGM2

Plasmid

#69239

Purpose

U6 driven SpCas9 sgRNA expression for TGM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TGM2 sgRNA (TGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126643

Purpose

Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_2xNLS_FnCpf1_MBP_protein_expression

Plasmid

#120801

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

2xNLS_FnCpf1

Use

CRISPR

Tags

MBP and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge