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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Publication

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Publication

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Tags

6xHis and OmpA3

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Publication

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Publication

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pRK5 FLAG human DEPTOR

Plasmid

#21334

Depositor

David Sabatini

Publication

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR (DEPTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

N204S and S389N mutations (numbering according to…

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pF146 pSOD1WTAcGFP1

Plasmid

#26407

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pET15b CnA CnB

Plasmid

#11787

Depositor

Anjana Rao

Publication

Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

Insert

CnA CnB (PPP3R1, PPP3CA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Mutation

"Opt" indicates the I-SceI coding seque…

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA4TO-mito-mCherry-10xGCN4_v4

Plasmid

#60914

Purpose

A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

mito-mCherry

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Publication

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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