We narrowed to 4,867 results for: gne
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Plasmid#172848PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B9
Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EPG
Plasmid#117978PurposeExpress EPG in mamalian cellsDepositorInsertElectromagnetic perceptive gene
UseTagsHIS and v5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKG139
Plasmid#69755PurposeRetroviral expression of CENP-CDepositorInsertCENP-C (CENPC Human)
UseRetroviralTagsmCherry-TEV-SExpressionMammalianMutationL341F, N570D relative to NP_001803.2 (numbering b…PromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2
Plasmid#71712PurposeMembrane scaffold protein (MSP), half of helix 4 truncatedDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF YFP (2XPDZ-YFP-LARG DH)
Plasmid#80408PurposeRecruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Scal-T2A-EGFP-Puro
Plasmid#234949PurposeDoxycycline-inducible mitochondria-localized ScaI expressing plasmidDepositorInsertScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-T2A-EGFP-Puro
Plasmid#234948PurposeDoxycycline-inducible mitochondria-localized ApaLI expressing plasmidDepositorInsertApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
UseTagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mCebpe
Plasmid#79076PurposeExpression of mouse CebpeDepositorInsertCebp epsilon (Cebpe Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.2_mTet3
Plasmid#79625PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.1_mTet3
Plasmid#79624PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM37-GFPnb-mCh
Plasmid#235666PurposeExpresses designed activator of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM37-GFPnb-mCh (PMCH Synthetic, Human)
UseTagsGFP nanobody and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mCebpa
Plasmid#79551PurposeExpression of mouse CebpaDepositorInsertCebp alpha (Cebpa Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
opto-E-cad GFP
Plasmid#203327PurposeExpresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cellsDepositorInsertPhotoswitchable - E-cadherin with GFP tag (CDH1 Human)
UseTagsGFPExpressionMammalianMutationAddition of Aslov2 domain at T133 of human E-cadh…PromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro
Plasmid#234950PurposeHuman codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only