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Plasmid#132651PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 55 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT2-HA-RAPGEF2(S1244A/S1248A)
Plasmid#110163PurposeExpression of human RAPGEF2 (S1244A/S1248A) in mammalian cellsDepositorInsertRAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
UseTagsHAExpressionMammalianMutationS1244A/S1248APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_1100
Plasmid#110117PurposeExpresses a 1100-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_900
Plasmid#110116PurposeExpresses a 900-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_700
Plasmid#110115PurposeExpresses a 700-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_500
Plasmid#110114PurposeExpresses a 500-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Cav2.2 DomI and DomII
Plasmid#89891PurposeMammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2DepositorInsertCacna1b (CACNA1B )
UseTagsExpressionMammalianMutationStop codon after amino acid 1154 (this is a trunc…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rabbit GFP Cav 2.2 pMT2
Plasmid#58737Purposeexpression of GFP tagged rabbit Cav2.2 in mammalian cellsDepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)
Plasmid#92402PurposeConstitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and No tag, cytosolic expressionExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
UseTags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianMutationPromoterCMV,&RhamnoseAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
UseTagsLckExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EA2, R57A, R59A pMT2
Plasmid#206122Purposeexpression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currentsDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationCav2.1-P1217fs, R57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT2 TIA1 RRM-CFP-FRBx5
Plasmid#103783Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-CFP-FRBx5
UseTagsCFPExpressionMammalianMutationPromoterSV40Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only