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lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a Lm-iPGM

Plasmid

#180242

Purpose

Bacterial expression plasmid for production of recombinant Leishmania mexicana iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Leishmania mexicana iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304-IRES-GFP

Plasmid

#176288

Purpose

pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMef2c-AHF-DEST (JDW 476)

Plasmid

#242577

Purpose

Gateway destination vector with the murine Mef2c anterior heart field promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDESTmycDDX17

Plasmid

#19876

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-EGFP

Plasmid

#188900

Purpose

Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

EGFP

Use

Lentiviral

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLV/eGFP-U6-sgRNA

Plasmid

#170544

Purpose

A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

ADAR2 deaminase domain (ADARB1 Human)

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLViP-LifeAct-iRFP670

Plasmid

#229699

Purpose

Lentiviral expression of LifeAct-iRFP670 in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-XPO1-ts1

Plasmid

#174286

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-XPO1-ts2

Plasmid

#174287

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHCD1E

Plasmid

#102956

Purpose

gene cloning of human CD1E

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

CD1E cDNA (CD1E Human)

Use

Cloning

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-IRES-Td

Plasmid

#176296

Purpose

pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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