We narrowed to 9,777 results for: 158

1. Lenti_AID-Dam-lamin B1

   Plasmid

   #119764

   Purpose

   Lentiviral plasmid for Mammalian Expression

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   AID-Dam-lamin B1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Mito-mRFP

   Plasmid

   #169001

   Purpose

   Express Mito (Su9)-mRFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
   

   Use

   Unspecified

   Tags

   mRFP

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_3xFlagEzh2 RNA binding mutant

   Plasmid

   #173718

   Purpose

   RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 RNA-binding mutant
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWP78-PVCpnf_pvc13-truncated

   Plasmid

   #198286

   Purpose

   Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-truncated
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR1068

   Plasmid

   #111092

   Purpose

   BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BlpI site disrupted

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-P3-evoCjCas9-TadCDd_gRNA

   Plasmid

   #202563

   Purpose

   P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-evoCjCas9-BE4max-BlastR

    Plasmid

    #194061

    Purpose

    Mammalian expression of evoCjCas9 CBE4max

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD2529-CAG-HA-mouse LRRC33

    Plasmid

    #180259

    Purpose

    Mouse full length LRRC33 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    LRRC33 (Nrros Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNTI827

    Plasmid

    #216635

    Purpose

    Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID-ERM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD2529-CAG-Flag-mouse TGFb1

    Plasmid

    #180260

    Purpose

    Mouse full length TGFβ1 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ1 (Tgfb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CMV-evoCjCas9-BlastR

    Plasmid

    #194059

    Purpose

    Mammalian expression of evoCjCas9 nuclease

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p416 25Q GPD

    Plasmid

    #1177

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAWP78-PVCpnf_pvc12-HiBiT

    Plasmid

    #198308

    Purpose

    Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc12-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYU-2-Env

    Plasmid

    #133997

    Purpose

    Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

    Depositor

    Himanshu Garg

    Article

    Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

    Insert

    Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR-flag-GFP-Intb1

    Plasmid

    #205193

    Purpose

    This vector encodes of a synCAM (Intb1) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Intb1 and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p416 103Q GPD

    Plasmid

    #1180

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pAS006

    Plasmid

    #211506

    Purpose

    AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLX307 LacZ

    Plasmid

    #117730

    Purpose

    Open reading frame vector encoding LacZ

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SRF WT pGEX4T

    Plasmid

    #121100

    Purpose

    Expression of SRF as a GST fusion protein

    Depositor

    Paul Herring

    Article

    Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAP003l2

    Plasmid

    #209748

    Purpose

    RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p416 72Q GPD

    Plasmid

    #1179

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only