We narrowed to 10,106 results for: HA

1. pGinB-8X(GGS)-dCas9-FLAG-NLS

   Plasmid

   #81205

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PROM_MpUBE2

   Plasmid

   #136109

   Purpose

   PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM_MpUBE2 Mapoly0106s0019
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. 5UTR_MpUBE2

   Plasmid

   #136110

   Purpose

   5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   5UTR_MpUBE2 Mapoly0106s0019
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCk2_spacer

   Plasmid

   #136072

   Purpose

   Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCTcon2-5INS_16

   Plasmid

   #102292

   Purpose

   Yeast Surface Display of 5Ins_16 scFV

   Depositor

   Sarel Fleishman

   Article

   Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

   Insert

   Insulin binding scFV
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-sod1

   Plasmid

   #107594

   Purpose

   Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   sod1 (sod1 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLNC Wnt-3aHA

   Plasmid

   #18030

   Depositor

   Jan Kitajewski

   Article

   Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

   Insert

   Wnt-3a (Wnt3a Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCk1_spacer

   Plasmid

   #136071

   Purpose

   Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1_nHA Spinophilin

    Plasmid

    #87122

    Purpose

    mammalian expression of Spinophilin

    Depositor

    Anthony Baucum

    Article

    Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

    Insert

    Spinophilin (PPP1R9B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCB-hDot1Lmut

    Plasmid

    #74174

    Purpose

    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    hDot1L 163 GSG to RCR mutation*

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCTcon2-5INS_EV3

    Plasmid

    #102293

    Purpose

    Yeast Surface Display of 5Ins_16_EV3

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Evolved Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE Hygro ST6GalNaC5

    Plasmid

    #26981

    Depositor

    Joan Massague

    Article

    Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

    Insert

    ST6GalNaC5 (ST6GALNAC5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOZ-C 16 E7

    Plasmid

    #13705

    Depositor

    Karl Munger

    Article

    Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pOZ-N 16 E7

    Plasmid

    #13706

    Depositor

    Karl Munger

    Article

    Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. TAL-L1-1.3-Ctrl

    Plasmid

    #102355

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p35S-PBE

    Plasmid

    #119772

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    NLS-APOBEC1-nCas9-UGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-nDsRedFL-miR9/9*

    Plasmid

    #177805

    Purpose

    The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TAL-L1-1.3-VP64

    Plasmid

    #103033

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3-VP64
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    HA and VP64

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR1326

    Plasmid

    #111131

    Purpose

    Expresses UBE2T.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUb dRxCas13d + RxCas13d guide RNA

    Plasmid

    #176304

    Purpose

    Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUB6-VSV-GS

    Plasmid

    #190781

    Purpose

    Expresses VSV-GS in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    VSV-GS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TAL-L1-2.4-VP64

    Plasmid

    #103039

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-2.4-VP64
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    HA and VP64

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-GoldenGate

    Plasmid

    #47388

    Purpose

    Expression plasmid for TALE proteins compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TAL-L1-1.3-KRAB

    Plasmid

    #103034

    Purpose

    [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3-KRAB
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    HA and KRAB

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA4TO OM-eLIR

    Plasmid

    #189004

    Purpose

    mitophagy-inducing adaptor protein

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    OM-eLIR
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSCV LDTS-LIRmut

    Plasmid

    #189001

    Purpose

    lipophagy-inducing adaptor protein, negative control

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-LIRmut
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS Wnt-3aHA

    Plasmid

    #17374

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-3aHA (Wnt3a Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

35. Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

    Plasmid

    #135462

    Purpose

    Neuronal expression of uFLARE TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
    

    Use

    AAV

    Tags

    HA, flag, VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3 NHE-RF

    Plasmid

    #11635

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    NHE-RF (NHERF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pUBC_HA_2xPP7-VP64

    Plasmid

    #68418

    Purpose

    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    PP7(deltaFG)
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    human UbC

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pClneoHA mouse Numb

    Plasmid

    #37012

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    Numb (Numb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains Numb aa 8-853

    Promoter

    CMV

    Available since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-nDsRedFL-miR124

    Plasmid

    #177806

    Purpose

    The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge