We narrowed to 25,242 results for: FER

1. pCry2PHR-mCh-RhoA

   Plasmid

   #42958

   Purpose

   Expresses optogenetically controlled RhoA

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3 basic Mut E2

   Plasmid

   #48748

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutated E-box2 sequence from CACGTT to GCTAGT

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-Hsp-Fluc-PGK-Rluc-mCherry

   Plasmid

   #191863

   Purpose

   Heat inducible Fluc reporter with constitutive Rluc

   Depositor

   Yingxiao Wang

   Article

   Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

   Inserts

   Firefly luciferase Fluc
   Renilla luciferase Rluc
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   HSPA7 promoter and PGK promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Cluc-RMA

   Plasmid

   #189624

   Purpose

   Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   Cypridina luciferase fused to Fc
   

   Use

   AAV and Luciferase

   Tags

   Cluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYDE009

   Plasmid

   #194151

   Purpose

   Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   tetR-tetP-dcas9-rrnB T1-T7Te
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetp

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127321

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-OT (wt TCF-4 sites)

   Plasmid

   #16558

   Depositor

   Bert Vogelstein

   Article

   Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

   Insert

   TCF-4 binding sites (TCF4 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   3 copies of wildtype Tcf-4 binding sites

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-GFP-WT1 +KTS N terminal HA

   Plasmid

   #217639

   Purpose

   Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 +KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ-pre-pro-alphaf-Btl2

    Plasmid

    #117664

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FMRP shRNA

    Plasmid

    #206074

    Purpose

    expression of FMRP RNA for gene silencing in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Ferron et al Nat Commun. 2014 Apr 7;5:3628. doi: 10.1

    Insert

    FMRP (Fmr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid

    #123362

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Firefly luciferase
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-OF (mutant TCF-4 sites)

    Plasmid

    #16559

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    TCF-4 binding sites (TCF4 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    3 copies of mutant Tcf-4 binding sites

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-FLIP1500

    Plasmid

    #16016

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    FLIP (CFLAR Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172316

    Purpose

    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EID1-pGex-2TK

    Plasmid

    #22689

    Depositor

    William Kaelin

    Article

    Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

    Insert

    E1A-like inhibitor of differentiation 1 (EID1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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