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pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Expression

Bacterial

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMRE-Himar-175

Plasmid

#185943

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Tags

FLAG tag

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKP1141

Plasmid

#160811

Purpose

Expression of TonB protein

Depositor

Kathleen Postle

Article

Gresock et al Front Microbiol. 2011 Oct 12;2:206. doi:

Insert

tonB (tonB Escherichia coli)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
lvl0 G10-90p

Plasmid

#153370

Purpose

Synthetic promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G10-90 promoter

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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L_EGFP_AC

Plasmid

#185971

Purpose

Expresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

Pl-LacO1-Egfp

Use

Synthetic Biology

Promoter

PlLacO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-134

Plasmid

#185925

Purpose

Himar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mOrange2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CMJ119

Plasmid

#69517

Purpose

pBAD eutE1-20- gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pHSG-PCNA1

Plasmid

#66125

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 1 (SSO_RS01995 Sulfolobus solfataricus P2)

Use

Cloning vector

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pINS-Neo

Plasmid

#31390

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

aph

Use

Cre/Lox

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)

Plasmid

#34865

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9- AsiA_m1.1

Plasmid

#158064

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 1.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m1.1

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO 9.6_bCas9-CP1010

Plasmid

#121498

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.15_bCas9-CP1249

Plasmid

#121500

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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