We narrowed to 4,942 results for: puc

1. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169667

   Purpose

   Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   vanillic acid-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   VanO2-PCMVmin

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-RiboJ-mTFP1-T7

   Plasmid

   #173225

   Purpose

   Encodes mTFP fluorescent protein. To be used in cell-free system

   Depositor

   Jim Haseloff

   Article

   Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

   Insert

   mTFP1
   

   Use

   Cell-free system

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGC-T7RibJ-RRvT

   Plasmid

   #173226

   Purpose

   Encodes RRvT fluorescent protein. To be used in cell-free system

   Depositor

   Jim Haseloff

   Article

   Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

   Insert

   RRvT
   

   Use

   Cell-free system

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

   Plasmid

   #178094

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + EBFP nick sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

   Plasmid

   #178095

   Purpose

   Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + EGFP nick sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDS_Cas9-NLS

   Plasmid

   #136121

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS_AtcoCas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4-Cquin_728

   Plasmid

   #176656

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLib6.4B-Agam_557

   Plasmid

   #176667

   Purpose

   Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLib6.4B-Aaeg_972

   Plasmid

   #176674

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLib6.4-Agam_557

    Plasmid

    #176653

    Purpose

    Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLib6.4B-Aalb_726

    Plasmid

    #176675

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLib6.4B-Aaeg_763

    Plasmid

    #176672

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLib6.4-Aaeg_972

    Plasmid

    #176660

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLib6.4-Cquin_801

    Plasmid

    #176657

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLib6.4B-Cquin_801

    Plasmid

    #176671

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLib6.4B-Cquin_728

    Plasmid

    #176670

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAT15488_HeF-ABE8e

    Plasmid

    #174126

    Purpose

    plasmid expressing HeF-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAT15487_evo-ABE8e

    Plasmid

    #174125

    Purpose

    plasmid expressing evo-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evo-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAT15486_HypaR661A-ABE8e

    Plasmid

    #174124

    Purpose

    plasmid expressing HypaR661A-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HypaR-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT15484_HF-ABE8e

    Plasmid

    #174122

    Purpose

    Expresses ABE8e fused with SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge