We narrowed to 24,218 results for: c-MYC

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_DT1

Plasmid

#136668

Purpose

Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_DT1

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_D1811

Plasmid

#136667

Purpose

Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1811

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_D1024

Plasmid

#136666

Purpose

Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1024

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA5_D4276

Plasmid

#136664

Purpose

Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D4276

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA5_D1126

Plasmid

#136663

Purpose

Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D1126

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR E25R

Plasmid

#119831

Purpose

Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25R

Tags

GST

Expression

Bacterial

Mutation

E25R

Promoter

T7

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S_S1966C

Plasmid

#98683

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-VMAc-CGFP

Plasmid

#70226

Purpose

Expresses C-terminal portion of GFP along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

eGFP

Tags

FKBP-rapamycin binding (FRB) domain of the mammal…

Expression

Mammalian

Mutation

Changed Ile 129 to Cys, Deleted amino acids 1-128

Promoter

CMV

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Article

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
ptHe-Tub2C-mScarlet

Plasmid

#201731

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2C tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta C

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo

Plasmid

#191398

Purpose

HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-7

Plasmid

#163135

Purpose

pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Nanobody, C-IgAP autotransporter

Expression

Bacterial

Promoter

P(rhaB)

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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CLYBL-Bxb1-LP-v2-TC

Plasmid

#194327

Purpose

CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro_FlnaABD-TS-16flag

Plasmid

#234868

Purpose

Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

Depositor

Michael Murrell

Article

Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

Insert

FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16

Use

Lentiviral

Tags

GFP, RFP, FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57-RFP-P2A-MCS (Neo)

Plasmid

#89182

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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