We narrowed to 10,106 results for: HA

1. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

   Plasmid

   #31480

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (ani target)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry has M9S, M16L to reduce background mCherr…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH4531

   Plasmid

   #132366

   Purpose

   Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Use

   Tags

   none

   Expression

   Worm

   Mutation

   Promoter

   Prgef-1

   Available since

   Sept. 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. TAL-L1-2.4-Ctrl

   Plasmid

   #103037

   Purpose

   Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-2.4
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   FLAG and HA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBW-m4046758

   Plasmid

   #145670

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pNOC-CRISPR

   Plasmid

   #100008

   Purpose

   Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BII-ChPtW-PLAGL2

   Plasmid

   #133380

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   PLAGL2 (PLAGL2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xFRB-myc

   Plasmid

   #20226

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. PB_TRE_AP4-PDGFR_EF1a_eBFP2

   Plasmid

   #186306

   Purpose

   Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP4-PDGFR
   eBFP2
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-nDsRedFL-miRNT

   Plasmid

   #177808

   Purpose

   A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-NT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHA-ΔN335-Kin1A

    Plasmid

    #31606

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN335 kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-335

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TAL-L1-Tf1-Ctrl

    Plasmid

    #103036

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-Tf1
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSH1/Sn-E-Fv'Fvls-E

    Plasmid

    #15274

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36) (FKBP1A Human)
    

    Use

    Tags

    HA epitope

    Expression

    Mammalian

    Mutation

    FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 AT1R DRY-AAY

    Plasmid

    #45759

    Depositor

    Robert Lefkowitz, Laszlo Hunyady

    Article

    Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

    Insert

    AT1R DRY-AAY (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed amino acids 125-126 from DR to AA

    Promoter

    CMV

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLPC-FLAG.HA.HA.Mm.RAP1

    Plasmid

    #184642

    Purpose

    Retroviral expression of mouse RAP1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    RAP1
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pShGSK3bE

    Plasmid

    #37526

    Depositor

    Jerry Crabtree

    Article

    Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

    Insert

    GSK3b (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SRa promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mouse Cavin-1

    Plasmid

    #50441

    Purpose

    expresses mouse Cavin-1 in mammalian cells

    Depositor

    Paul Pilch

    Article

    Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

    Insert

    Cavin-1 (Cavin1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pEGFP-Akt(R25C)

    Plasmid

    #39532

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt(R25C) (Akt1 Mouse)
    

    Use

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

22. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid

    #186307

    Purpose

    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P9-PDGFR
    mPlum
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid

    #186308

    Purpose

    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP10-PDGFR
    mOrange
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid

    #186305

    Purpose

    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P3-PDGFR
    iRFP670
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3-Control-miR-20-Control

    Plasmid

    #21169

    Depositor

    Joshua Mendell

    Article

    O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

    Insert

    miR-20 Sensor (MIR20A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sensor construct insert has two miR-20 binding si…

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3158 pSFFV-neo Bad cDNA

    Plasmid

    #8753

    Depositor

    Stanley Korsmeyer

    Article

    Yang et al Cell 1995 Jan 27;80(2):285-91.

    Insert

    BAD (Bad Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBRIT Pax7 216C

    Plasmid

    #17523

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-215

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pUb LwaCas13a + LwaCas13a Guide RNA

    Plasmid

    #176307

    Purpose

    Expresses LwaCas13a and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    LwaCas13a
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E656G, I1060V, D1076G- please see depositor comme…

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TAL-L1-1.3-DEL

    Plasmid

    #103035

    Purpose

    [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by DEL domian

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3-DEL
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    DEL and HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pM214

    Plasmid

    #173216

    Purpose

    Expresses MCP-mCherry-GFP11-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    MCP-mCherry-GFP11-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGBW-m4046926

    Plasmid

    #145663

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. hPlekhm1_pIRES puro Glue

    Plasmid

    #73453

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBRIT Pax7 N281

    Plasmid

    #17524

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 281 amino acids

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

36. GAL HA25QDProCFPp303

    Plasmid

    #15578

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

37. GAL HA103QDProCFPp303

    Plasmid

    #15579

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pTPTP alpha (S204A)

    Plasmid

    #17694

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S204A (PTPRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S204A

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pTH736-CEN-staHIS3

    Plasmid

    #38228

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Synthetic, Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBRIT Pax7 N391

    Plasmid

    #17525

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 391 amino acids

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only