We narrowed to 19,551 results for: Cre

1. pCRISPathBrick

   Plasmid

   #65006

   Purpose

   E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive native promoters

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR-StAR4B (Lenti)

   Plasmid

   #222691

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF12J

   Plasmid

   #42943

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   EN+ Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   AgeI-BclI fragment swapped with nt 1927-3708 from…

   Promoter

   CMV MIE, L1 5'UTR

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

6. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-gst-xr

   Plasmid

   #214761

   Purpose

   Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

   Depositor

   Pimchai Chaiyen

   Article

   Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

   Insert

   glutathione S-transferases fused with xylose reductase
   

   Tags

   Glutathione S-transferases

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_SC_4.26_STARR-seq

   Plasmid

   #220220

   Purpose

   AAV-STARR-seq screening vector with 4.26 promote

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   4.26

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMax_mOrange_A215C

    Plasmid

    #177828

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_A215C
    

    Expression

    Mammalian

    Mutation

    A215C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPseq-BFP-backbone

    Plasmid

    #85707

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRISPR-StAR1 (Retro)

    Plasmid

    #222687

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPR-StAR2 (Retro)

    Plasmid

    #222688

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRISPR-StAR3 (Retro)

    Plasmid

    #222689

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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