We narrowed to 18,265 results for: Met;

1. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast CG1358

   Plasmid

   #218541

   Purpose

   Drosophila CG1358 cDNA (human codon optimized)

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CG1358 (HisT Fly)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. plentiCRISPR-v2 Puro SPNS1_sg2

   Plasmid

   #218531

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. plentiCRISPR-v2 Puro SPNS1_sg1

   Plasmid

   #218530

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKG-GW1-SRT1

   Plasmid

   #203171

   Purpose

   Yeast expression vector for yeast SRT1

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Insert

   SRT1 (SRT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2x3APWWP_K299E_D333A-IFP2.0C

   Plasmid

   #215753

   Purpose

   Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2x3APWWP_K299E_D333A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF702

   Plasmid

   #209650

   Purpose

   Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

   Depositor

   Bastian Molitor

   Article

   Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

   Inserts

   Downstream homologous region
   thermostable neomycin phosphotransferase gene
   Upstream homologous region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLNRD1_35-345_eGFP

   Plasmid

   #208881

   Purpose

   Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acid sequence 35-345

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mito-PDCD10-mScarlet-I

   Plasmid

   #208887

   Purpose

   Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM3 (PDCD10 Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   PDCD10 targeted to the mitonchondria

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TLNRD1_5H_eGFP

    Plasmid

    #208861

    Purpose

    Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 5 helix bundle (TLNRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acid 142-273

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-TRE-IEo

    Plasmid

    #201992

    Purpose

    AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Tight TRE

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKG-GW1-RER2

    Plasmid

    #203170

    Purpose

    Yeast expression vector for yeast RER2

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    RER2 (RER2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKG-GW1-GlcisPT

    Plasmid

    #203172

    Purpose

    Yeast expression vector for Giardia cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    UPPS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-RAPH1Δ536-587

    Plasmid

    #194865

    Purpose

    Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 536-587 deleted

    Promoter

    CMV

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_VNp-mNeongreen-Uricase

    Plasmid

    #182417

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-uricase
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_mNeongreen-StefinA

    Plasmid

    #182418

    Purpose

    Bacterial expression of mNeongreen-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-stefin (CSTA Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge