We narrowed to 4,117 results for: SPL

Showing: 4001 - 4020 of 4117 results

Results per page:

Sort results by:

pMAL-MBP-FRB-RhoA/N12

Plasmid

#214298

Purpose

Express split-RhoA fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-RhoA fragment fused with FRB (RHOA Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b-RhoA/13C-FKBP-MBP

Plasmid

#214299

Purpose

Express split-RhoA fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-RhoA fragment fused with FKBP (RHOA Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

RhoA-Q63L

Promoter

T7

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

Plasmid

#208110

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, Lmna sgRNA1, scaffold

Use

AAV

Promoter

U6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

Plasmid

#209776

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

Nluc, sgSTK4i, mRuby2 (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-620-I612AL613A-GB1_AB

Plasmid

#148358

Purpose

Bacterial Expression of DmMextli_577-620-I612AL613A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620-I612AL613A (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-620-Y581AL586AL587A-GB1_AB

Plasmid

#148359

Purpose

Bacterial Expression of DmMextli_577-620-Y581AL586AL587A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620-Y581AL586AL587A (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640-F625EL631E-GB1_AB

Plasmid

#148361

Purpose

Bacterial Expression of DmMextli_577-640-F625EL631E

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-F625EL631E (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640-I612AL613A-GB1_AB

Plasmid

#148363

Purpose

Bacterial Expression of DmMextli_577-640-I612AL613A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-I612AL613A (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640-M605D-GB1_AB

Plasmid

#148364

Purpose

Bacterial Expression of DmMextli_577-640-M605D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-M605D (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640-Y581AL586AL587A-GB1_AB

Plasmid

#148365

Purpose

Bacterial Expression of DmMextli_577-640-Y581AL586AL587A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-Y581AL586AL587A (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-640-YLLILtoAAAAA-GB1_AB

Plasmid

#148366

Purpose

Bacterial Expression of DmMextli_577-640-YLLILtoAAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-YLLILtoAAAAA (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_DRD2-NTEV-TCS-GV-2xHA

Plasmid

#194362

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD2-NTEV-TCS-GV-2xHA (DRD2 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NODAL-MYC-DYK-N72A-N199A

Plasmid

#115252

Purpose

For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Tags

MYC-DYK

Expression

Mammalian

Mutation

N72A, N199A

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2406-Tier1-PhCMV-NTEV

Plasmid

#169501

Purpose

Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven N-terminal fragment of split tobacco etch virus protease

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA T7-Gnat1 Mut-3UTR

Plasmid

#184027

Purpose

Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

TAG sites in the 3'-UTR are mutated to TGA

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB

Plasmid

#148357

Purpose

Bacterial Expression of DmMextli_577-620-I583RS590R

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620-I583RS590R (mxt Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

Plasmid

#120295

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRMT9 deltaC 350 (stop)

Plasmid

#67612

Purpose

bacterial expression of GST-PRMT9 missing residues 351-845

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codons placed at aa 351 and 352 to create C …

Promoter

tac

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only