We narrowed to 24,461 results for: nes

1. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 shPIK3CD.v2 puro

   Plasmid

   #58707

   Purpose

   Lentiviral shRNA vector for knockdown of human PIK3CD

   Depositor

   Kevin Janes

   Article

   Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Insert

   shPIK3CD (PIK3CD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pSTAP-seq_fly-tj

   Plasmid

   #86386

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster tj enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGL3-Ogt (-178 to +233 bp)

   Plasmid

   #79266

   Purpose

   To identify the Cis and Trans acting elements that control Ogt gene transcription.

   Depositor

   Steven Jones

   Article

   Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

   Insert

   Ogt promoter element (Ogt Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

5. K349CLM-6His 215/347

   Plasmid

   #24451

   Depositor

   Ron Vale

   Article

   Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

   Promoter

   Available since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. A337C/Y344C

   Plasmid

   #24434

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pDGE77

   Plasmid

   #80579

   Purpose

   recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:PAT-tnos
   p2x35S:Cas9 D10A-tnos
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE78

   Plasmid

   #80580

   Purpose

   recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:nptII-tmos
   pPcUbi:Cas9 D10A-tnos
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE79

   Plasmid

   #80581

   Purpose

   recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:PAT-tnos
   pPcUbi:Cas9 D10A-tnos
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. K222C/E334C

    Plasmid

    #24435

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

11. K420CLM RP

    Plasmid

    #24443

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCINTBeta1

    Plasmid

    #14067

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PstI-BglI fragment was blunt end cloned and EcoRI…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. CLM RP KGF Mut 1

    Plasmid

    #24166

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Promoter

    Available since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pX458-SLC35A1

    Plasmid

    #239952

    Purpose

    Encodes gRNA for human SLC35A1

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    SLC35A1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-MFSD6

    Plasmid

    #239951

    Purpose

    Encodes gRNA for human MFSD6

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2-ICAM-5-Puro

    Plasmid

    #239949

    Purpose

    CRISPR KO of human ICAM-5

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    ICAM-5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2-MFSD6-Puro

    Plasmid

    #239950

    Purpose

    CRISPR KO of human MFSD6

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid

    #239448

    Purpose

    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

    Depositor

    Alexis Komor

    Inserts

    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BFP: T65S, S72S, V145F, K206K, H231L

    Promoter

    EF1alpha, U6, and mU6

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. JD179

    Plasmid

    #229496

    Purpose

    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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