We narrowed to 6,266 results for: wus

1. PMP34-mApple

   Plasmid

   #214417

   Purpose

   Peroxisome localization of PMP34 fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_Efs_hSpCas9_NLS_FLAG-SV40

   Plasmid

   #97307

   Purpose

   AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   AAV and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR v2 puro hGSDME gRNA1

   Plasmid

   #223519

   Purpose

   knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-puro-mGSDME D270A

   Plasmid

   #223518

   Purpose

   Expresses mGSDME D270A in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D270A

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

   Plasmid

   #78607

   Purpose

   A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short; U6

   Available Since

   Sept. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

7. LentiCRISPR v2 RB1 sgRNA #1

   Plasmid

   #202516

   Purpose

   Expressing Cas9 and sgRNA targeting human RB1 gene

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   RB1 sgRNA (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV_Actb HR donor

   Plasmid

   #97317

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-NES-NIR-GECO1

   Plasmid

   #113683

   Purpose

   Express NIR-GECO1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NES-NIR-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-CMV-RPS4X-3xHA plasmid

    Plasmid

    #192246

    Purpose

    overexpression vector of RPS4X-3XHA

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    RPS4X CDS without stop coden (RPS4X Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-hSyn-Galpha-s ONE-GO

    Plasmid

    #214371

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGLOW39

    Plasmid

    #173068

    Purpose

    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    mScarlet-I-C1^SEC^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mScarlet

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-p53DD

    Plasmid

    #196582

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CMV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-pTau nanobody (A2) with sortase tag

    Plasmid

    #233218

    Purpose

    Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv 2023.10.24.563674

    Insert

    Anti-pTau nanobody (A2)
    

    Use

    Tags

    Sortase tag, His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX4T1_GST-PLproCS-MBP

    Plasmid

    #169195

    Purpose

    To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    GST-PLproCS-MBP
    

    Use

    Tags

    GST-MBP fusion (PLpro-cleavable) and His6 (intern…

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-hAID-BE3

    Plasmid

    #113417

    Purpose

    Expresses hAID-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hAID-BE3 (AICDA S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5'-3xFLAG Kdm1a-exon minimal CMV pCDNA5

    Plasmid

    #212054

    Purpose

    LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a-exon (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. JWW-2 human chimeric monoclonal antibody

    Plasmid

    #66749

    Purpose

    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-2 Heavy Chain
    JWW-2 Light Chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 and rEF1

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV.CBA.YFP.miR-E_shControl

    Plasmid

    #180386

    Purpose

    Producing AAV that encodes negative control shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shControl
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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