4,142 results

1. pMXs-hNANOG (Plath)

   Plasmid

   #18115

   Depositor

   Kathrin Plath

   Article

   Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

   Insert

   Nanog homeobox (NANOG Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1(-) mouse C/EBP delta

   Plasmid

   #12559

   Depositor

   Peter Johnson

   Article

   McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

   Insert

   CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S2G introduced during cloning

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. FCK-Halo-GFP

   Plasmid

   #14750

   Purpose

   Lentiviral expression of Halo-GFP for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al PLoS One. 2007 Mar 21;2(3):e299.

   Insert

   Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N. pharaonis halorhodopsin is mammalian codon-opt…

   Promoter

   CaMKIIa

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAd-Track Flag-SIRT1

   Plasmid

   #8438

   Depositor

   Pere Puigserver

   Article

   Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-CRY2-CreN

   Plasmid

   #26888

   Purpose

   Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2-CreN (cre, CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 19-104 of Cre

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Stat3 S727A pRc/CMV

   Plasmid

   #8708

   Depositor

   Jim Darnell

   Article

   Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

   Insert

   Stat3 S727A (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S727A (exhibits o serine phosphorylation either c…

   Promoter

   Available since

   Aug. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHB9-EGFP (Hb9 promoter) [TJ#96]

   Plasmid

   #16275

   Depositor

   Thomas Jessell

   Article

   Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

   Insert

   HB9 promoter
   

   Use

   Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5 TGF beta type I receptor (T202D) Flag

   Plasmid

   #14833

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Threonine 202 mutated to aspartate. Constitutivel…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 TGF beta type I receptor Flag

   Plasmid

   #14831

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMLM292

    Plasmid

    #21873

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMLM290

    Plasmid

    #21872

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC57 APEX

    Plasmid

    #40306

    Purpose

    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APEX (Synthetic)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    K14D, W41F, E112K (relative to wild-type)

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

    Plasmid

    #19326

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::twk-18(cn110)cDNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Kunihiko Naito, Wataru Fujii

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge