4,180 results

1. pMXs-Klf4-IP

   Plasmid

   #15920

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. myc-Rictor

   Plasmid

   #1860

   Purpose

   Please see Addgene item #11367 for an updated version of this plasmid.

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Rictor (RICTOR Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAL149

   Plasmid

   #22275

   Purpose

   Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB(1-908) (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mCherry-Kras4BCT (prenylation sequence)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. 811 pSG5L HA PTEN C124S

   Plasmid

   #10744

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C124S

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIREShyg-PA-GFP-alpha Tubulin

   Plasmid

   #12296

   Depositor

   Patricia Wadsworth

   Article

   Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   PA-GFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. OCT4-eGFP-PGK-Puro

   Plasmid

   #31937

   Purpose

   Oct4 targeting donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR/pTER+ (430-1)

   Plasmid

   #17453

   Purpose

   Gateway entry vector for shRNA expression under H1/TO promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   RNAi; Entry vector

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-ChETA-EYFP

   Plasmid

   #26967

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T

   Promoter

   CaMKIIa

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS302

    Plasmid

    #11925

    Depositor

    Brian Sauer

    Article

    Sauer et al Methods Enzymol. 1993;225:890-900. doi:

    Insert

    STOP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Expression

    Mammalian

    Mutation

    P31S

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. RNT1-GFP

    Plasmid

    #17708

    Depositor

    Hal Dietz

    Article

    Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

    Insert

    rent1 (UPF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUW-SOK

    Plasmid

    #20327

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

    Plasmid

    #9031

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M T308A S473A (AKT1 Human)
    

    Expression

    Mammalian

    Mutation

    K179M T308A S473A

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA cyclin D1 HA T286A

    Plasmid

    #11182

    Depositor

    Bruce Zetter

    Article

    Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

    Insert

    cyclin D1 T286A (CCND1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T286A. Mutant that cannot be degraded via the u…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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