174,131 results

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pOSIP-KO (KanR, 186)

Plasmid

#45985

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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beta-arrestin2 mYFP

Plasmid

#36917

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin2 (Arrb2 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Article

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del1

Plasmid

#32062

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Del1(E746-A750)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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hrGFP-Keap1

Plasmid

#28025

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

Keap1 (KEAP1 Human)

Tags

hrGFP

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ALK

Plasmid

#23917

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALK (ALK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R280K

Plasmid

#22933

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

R280K mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
Flag-HA-USP16

Plasmid

#22595

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP16 (USP16 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe-hygro PyMT

Plasmid

#22305

Depositor

Filippo Giancotti

Article

Pylayeva et al J Clin Invest. 2009 Feb . 119(2):252-66.

Insert

Polyoma Middle T

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only
hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASx beta

Plasmid

#15210

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx beta (PIAS2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pFLAG-GATA2

Plasmid

#1418

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-FF3

Plasmid

#11662

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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