We narrowed to 7,531 results for: Ski

1. pSLQ10714

   Plasmid

   #183959

   Purpose

   U6-crKlf4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Klf4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-sgRNA_Ago1_1

   Plasmid

   #73533

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-sgRNA_Ago1_2

   Plasmid

   #73534

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pCIBN-EYFP

   Plasmid

   #103815

   Purpose

   Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-pr-mEos4b (mEosFP4b-V69T)

   Plasmid

   #99226

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos4b
   

   Expression

   Bacterial

   Mutation

   V69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-gp120(BaL)-CD4

   Plasmid

   #98957

   Purpose

   Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   HIV-1 (BaL) gp120
   

   Tags

   CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-PGAM1_sgRNA2

   Plasmid

   #201615

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PGAM1 (PGAM1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TXD13-CD4d3+4-bio

   Plasmid

   #73108

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding TXD13 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   TXD13 (TMX4 Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. bio-CD4d3+4-ADL1

   Plasmid

   #73090

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1 with bio-rat CD4d3+4

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   ADL1 (NCEH1 Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1 FRB-myc-RagA

    Plasmid

    #112740

    Purpose

    express FRB-myc-RagA

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagA (RRAGA Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-iCre-2A-venus

    Plasmid

    #182499

    Purpose

    Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    iCre-2A-Venus
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    fragment of histidine decarboxylase gene promoter…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Supt6h-SH2

    Plasmid

    #46518

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Supt6h (SUPT6H Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1317-1441

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-TPD52

    Plasmid

    #172452

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIBN-mCherry

    Plasmid

    #103816

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arp2_pLib

    Plasmid

    #173676

    Purpose

    Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    Arp2 (ACTR2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 FRB-myc-RagC

    Plasmid

    #112741

    Purpose

    express FRB-myc-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Nck-SH2

    Plasmid

    #46457

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nck (NCK1 Human)
    

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 275-377

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX ShcA-SH2

    Plasmid

    #46486

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcA (SHC1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 476-584

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO_CEP41_HaloTag9

    Plasmid

    #169331

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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