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Plasmid#135409PurposeE. coli 'blaTEM-1 (without signal sequence) that can be used as a reporterDepositorInsertblaTEM-1
UseTagsExpressionBacterialMutationPromoterUnknownAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM261
Plasmid#128411PurposeVanillic acid inducible production of lysostaphin. LsyT with no signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseTagsExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK013-3a-Invertase-αMFΔ
Plasmid#84972PurposeType 3a, Invertase followed by αMFΔ - secretion signalDepositorInsertInvertase followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET-PesaR-Plux
Plasmid#47659PurposePesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-P
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box downstream of the original esa…PromoterPesaR-PAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFF749
Plasmid#61452PurposeIPTG-inducible msd_kanR(ON)_dRTDepositorInsertmsd_kanR(ON)_dRT
UseSynthetic BiologyTagsExpressionMutationD197A D198APromoterpLacOAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPTK014-3a-Killer-αMFΔ
Plasmid#84973PurposeType 3a, Killer followed by αMFΔ - secretion signalDepositorInsertKiller followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK011-3a-SA-αMFΔ
Plasmid#84970PurposeType 3a, Serum albumin followed by αMFΔ - secretion signalDepositorInsertSerum albumin followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWp7-12
Plasmid#72744PurposepFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]DepositorInsertUBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHIS2-hsSTAM1 (1-143)
Plasmid#21498DepositorInsertphis2- STAM1(1-143) (STAM Human)
UseTags6x HisExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPTK012-3a-Inulinase-αMFΔ
Plasmid#84971PurposeType 3a, Inulinase followed by αMFΔ - secretion signalDepositorInsertInulinase followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Dlux
Plasmid#47657PurposePesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-D
UseSynthetic BiologyTagsExpressionBacterialMutationAdded an additional esa box upstream of -35 site …PromoterPesaR-DAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pICH78141
Plasmid#50293DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (Oryza sativa)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM5331
Plasmid#50294DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFF761
Plasmid#61455PurposeaTc-inducible SCRIBE_galK(OFF)DepositorInsertSCRIBE_galK(OFF)
UseSynthetic BiologyTagsExpressionMutationPromoterpTetOAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVCS1
Plasmid#231615PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS9
Plasmid#231623PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS8
Plasmid#231622PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS6
Plasmid#231620PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS5
Plasmid#231619PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS4
Plasmid#231618PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS3
Plasmid#231617PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS2
Plasmid#231616PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2837
Plasmid#231676PurposeExpresses a rabbit IgH signal peptide fused to GFP in mammalian cellsDepositorInsertRabbit IgH-sfGFP
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
TMPRSS3:72-454_CO
Plasmid#220880PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS3 (TMPRSS3 Human)
UseBaculovirus expresssionTags8xHis and melittin signal sequenceExpressionMutationPromoterPolyhedrinAvailable sinceSept. 30, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
myr-palm-Gamillus(2x)
Plasmid#197921PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MTS-jAspSnFR3
Plasmid#203487PurposeMitochondrial directed jAspSnFR3 in lentiviral expression vectorDepositorInsertMTS-jAspSnFR3
UseLentiviralTagsMitochondrial localization signalExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPI-mGreenLantern(2x)
Plasmid#197917PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-mGreenLantern-mGreenLantern
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPI-Gamillus(2x)
Plasmid#197915PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only