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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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TRBV14

Plasmid

#123449

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV14 (TRBV14 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV2

Plasmid

#123409

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV2 (TRBV2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV19

Plasmid

#123391

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV19 (TRAV19 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV8-4

Plasmid

#123377

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-4 (TRAV8-4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.1

Plasmid

#111066

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Y1175F-VEGFR2-mCherry

Plasmid

#139608

Purpose

Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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TRBV6-5

Plasmid

#123425

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV6-5 (TRBV6-5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-diLACCO1

Plasmid

#208102

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RON-V5/HIS

Plasmid

#201983

Purpose

expression of human RON receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RON receptor tyrosine kinase (MST1R Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSems StayGold-2xFYVE

Plasmid

#222948

Purpose

Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-WPRE

Plasmid

#130992

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-mRuby3-MLC-IRES-Neo

Plasmid

#222635

Purpose

Reporter for activated NM2

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mRuby3-MLC (MYL9 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

Plasmid

#175563

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL1

Tags

Citrine

Expression

Plant

Promoter

RUBY

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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TRAV16

Plasmid

#123389

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV16 (TRAV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#123386

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV13-1 (TRAV13-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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