We narrowed to 4,995 results for: mcs

1. B0033m_BC

   Plasmid

   #66021

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. B0034m_BC

   Plasmid

   #66022

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. MSCV-N MCV ER

   Plasmid

   #37860

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Sabrina Spencer, Tobias Meyer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPonA-BI-Gl NORM-LacZA TER- LacZB

   Plasmid

   #86212

   Purpose

   beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

   Depositor

   Robert Singer

   Article

   Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

   Insert

   beta-Globin (HBB, Hbb Mouse, Human)
   

   Use

   Ponasterone a inducible promoter

   Tags

   lacZA or lacZB

   Expression

   Mammalian

   Mutation

   39TER in beta Globin in MCS II

   Promoter

   Ponasterone A inducible

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-KTN1-WPRE-UbC-Emerald

   Plasmid

   #225941

   Purpose

   Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Evan Reid, Richard Eva

   Inserts

   KTN1 (KTN1 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2-SynTetOff-palGFP

    Plasmid

    #190232

    Purpose

    AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    palmitoylation signal sequence

    Expression

    Mammalian

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTSin EGFP

    Plasmid

    #127695

    Purpose

    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH_Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65258

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid

    #196415

    Purpose

    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH_CMV_TFAM_mKate2

    Plasmid

    #167331

    Purpose

    Expresses mKate2 fused to full length TFAM in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNAHA

    Plasmid

    #44169

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    hemagglutinin gene
    neuraminidase gene
    

    Expression

    Mammalian

    Mutation

    T55A

    Promoter

    CMV enhancer/human Ferritin light chain promoter …

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-mOC-STAMP-GFP

    Plasmid

    #73579

    Purpose

    Lentivirus expression of mOC-STAMP

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-TRE-FLEX-eNpHR-EYFP

    Plasmid

    #89876

    Purpose

    To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    eNpHR=EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV asyn WT

    Plasmid

    #36055

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein WT (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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