We narrowed to 8,320 results for: Set

1. pMo130-TelR

   Plasmid

   #50799

   Purpose

   Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

   Depositor

   Kim Lee Chua

   Article

   Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

   Insert

   tellurite-resistance cassette
   

   Use

   Suicide vector

   Tags

   Expression

   Mutation

   Promoter

   PCs12

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pRPS5a

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC018 - LshCas13a from Leptotrichia shahii

   Plasmid

   #91908

   Purpose

   Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LshCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS mRFP

   Plasmid

   #91765

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

   Insert

   monomeric Red Fluorescent Protein1
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MXS_CMV::ZeoR-bGHpA

   Plasmid

   #62441

   Purpose

   MXS_chaining vector with CMV::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaS

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. tubP-FRT>GAL80-6-FRT>

    Plasmid

    #63173

    Purpose

    ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "Flp-Out" GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pre_II_CS3_7653_v2

    Plasmid

    #126855

    Purpose

    7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7653_v2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179012

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

    Plasmid

    #119903

    Purpose

    Bxb1 integration plasmid for cuO array with Puro selection cassette

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    cuOx144 array
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TET

    Plasmid

    #20909

    Purpose

    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. miReporter-PGK

    Plasmid

    #82477

    Purpose

    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

    Plasmid

    #112925

    Purpose

    Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6 promoter, gRNA-SAM expression cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMVV102

    Plasmid

    #215308

    Purpose

    Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PTac

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMVV103

    Plasmid

    #215309

    Purpose

    Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pR26 GFP Dest

    Plasmid

    #74283

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDonor_RB-TnV2

    Plasmid

    #213907

    Purpose

    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCI SMN2

    Plasmid

    #72287

    Purpose

    SMN2 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN2 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains exons 6-7-8 including introns, multiple …

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSCARS1

    Plasmid

    #174868

    Purpose

    Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

    Depositor

    Zengyi Shao

    Article

    Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

    Insert

    ARS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid

    #66707

    Purpose

    expresses a single gRNA and VP64-dCas9-VP64

    Depositor

    Charles Gersbach

    Inserts

    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hU6 and hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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