We narrowed to 26,528 results for: CAL;

1. Cav2.2 HA EIVK (E1658K) pcDNA3

   Plasmid

   #206066

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658K

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.2 HA EIVK (E1658K) pCAGGS

   Plasmid

   #206096

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658K

   Promoter

   CMV/B-actin

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pb-miRE-tre-3xFLAG-hnRNPU∆RGG

   Plasmid

   #196088

   Purpose

   Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAF-A (Hnrnpu Mouse)
   

   Use

   Tags

   N-terminal 3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   removed last 154 amino acids on cterminal end

   Promoter

   TRE

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

   Plasmid

   #196515

   Purpose

   Expression of Myc-Eps8L2_1-496

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-496 (EPS8L2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-NLS-YFP-CCTc

   Plasmid

   #184327

   Purpose

   Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTc (CACNA1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SspB-mCherry-DHPH-ITSN1

   Plasmid

   #176103

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 FLAG membrane-bound lipin-1 (delta200)

   Plasmid

   #120278

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG and in-frame fusion with the delta200 Golgi-…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEAHISPSVORF126

   Plasmid

   #97021

   Purpose

   Expressing Pyrobaculum spherical virus orf126 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 126
   

   Use

   Tags

   N-ter TEV protease cleavable 6HIs

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAHISPSVORF239

   Plasmid

   #97025

   Purpose

   Expressing Pyrobaculum spherical virus orf239 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 239
   

   Use

   Tags

   N-ter TEV protease cleavable 6HIs

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEAHISPSVORF165A

    Plasmid

    #97024

    Purpose

    Expressing Pyrobaculum spherical virus orf165a protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 165a
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEAHISPSVORF137

    Plasmid

    #97023

    Purpose

    Expressing Pyrobaculum spherical virus orf137 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 137
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEAHISPSVORF131

    Plasmid

    #97022

    Purpose

    Expressing Pyrobaculum spherical virus orf131 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 131
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mBFP-APP-mGFP

    Plasmid

    #196694

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid

    #104487

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV Marina-T2A-nls-mCherry

    Plasmid

    #74216

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-APPP1-mGFP

    Plasmid

    #196705

    Purpose

    As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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