We narrowed to 16,827 results for: puromycin

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA3_GAL4UAS-Luciferase reporter

Plasmid

#64159

Purpose

Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3 for GAL4UAS-Luciferase reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPDS1-mEGFP-IRES-puro2b

Plasmid

#26464

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pPDS1 (PTTG1 Human)

Tags

mEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pGATOR

Plasmid

#64102

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H1_2

Plasmid

#36343

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSR-puro-Mff1 shRNA

Plasmid

#37247

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Mff1 (MFF Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pHBS1084 GFP-TDP43 deltaCTD

Plasmid

#118798

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

Deletion of CTD in full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2-puro

Plasmid

#44111

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
ps226

Plasmid

#133557

Purpose

RNAi construct for knock-down of CDKN1A (Cip1, p21)

Depositor

David Livingston

Article

Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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YSEHPTFTSQY_epitope

Plasmid

#165016

Purpose

Expression of CMV UL83 (363-373) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL83 (363-373)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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hADAM10 siRNA pSIREN-Retro-Q

Plasmid

#19140

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 siRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pHAGE- TRE-DEST-CBioTAP

Plasmid

#53569

Purpose

Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA2

Plasmid

#22508

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA2 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2puro-Tyr

Plasmid

#170293

Purpose

A knock-out vector for the mouse tyrosinase.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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MO70 Orai1 N223A

Plasmid

#21663

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

N223A

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV-Pidd1(T788D)

Plasmid

#60530

Purpose

Retroviral expression of Pidd1 T788D phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Aspartic acid*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only