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We narrowed to 9,474 results for: BLI;

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Showing: 8201 - 8240 of 9474 results

  1. pMamCol1

    Plasmid
    #215184
    Purpose
    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli
    Depositor
    Krishna Kishore Inampudi
    Insert
    tsPurple
    Use
    AAV, CRISPR, and Synthetic Biology ; Recombinant …
    Tags
    SNAC-StrepII tag
    Expression
    Bacterial and Mammalian
    Promoter
    TetON 3G Bidirectional for Mammalian and Trc with…
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215184-plasmid-map-sequence-id-430375

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  4. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  5. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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  6. pWR54

    Plasmid
    #203330
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203330-plasmid-map-sequence-id-417329

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  7. pWR24

    Plasmid
    #202771
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coNAT
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202771-plasmid-map-sequence-id-409762

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  8. pWR16

    Plasmid
    #202711
    Purpose
    integrating bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202711-plasmid-map-sequence-id-409745

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  9. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  10. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid
    #203805
    Purpose
    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Llgl1 (Llgl1 Mouse)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203805-plasmid-map-sequence-id-401577

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  11. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid
    #196973
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GFP and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196973-plasmid-map-sequence-id-388919

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  12. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid
    #196976
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GST and SNAP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196976-plasmid-map-sequence-id-388922

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  14. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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  15. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

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  17. pENTR4 CDS A. thaliana PARP1

    Plasmid
    #175818
    Purpose
    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Insert
    PARP1 (PARP1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Mutation
    one silent mutation, annotated in GenBank file
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175818-plasmid-map-sequence-id-346172

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  19. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid
    #168770
    Purpose
    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Mutation
    See Depositor Comments Below
    Available Since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    168770-plasmid-map-sequence-id-337553

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  20. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

    Plasmid
    #141452
    Purpose
    B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Cdk6 (Cdk6 Mouse)
    Use
    Mouse Targeting; Dre / rox
    Tags
    C-terminal fusion of T2A-TagRFPT-dre-PBD*
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    141452-plasmid-map-sequence-id-315092

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  21. psiCHECK2 3'UTR GAC

    Plasmid
    #110401
    Purpose
    Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110401-plasmid-map-sequence-id-227284

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  22. pUC19 KGA HA5'-mKO2-3'

    Plasmid
    #110406
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Promoter
    none
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110406-plasmid-map-sequence-id-218847

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  28. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107300
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107300-plasmid-map-sequence-id-204631

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  29. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107299
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107299-plasmid-map-sequence-id-204615

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  30. Halo-Tev-Akt3 C119S-2X Flag

    Plasmid
    #87608
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    AKT3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    cysteine 119 residue mutated to serine
    Available Since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87608-plasmid-map-sequence-id-197921

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  31. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  32. pGS1623

    Plasmid
    #63795
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Twin-Strep tag followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Twin-Strep
    Promoter
    none
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63795-plasmid-map-sequence-id-110439

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  33. pGS1322

    Plasmid
    #63792
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Twin-tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Twin-tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63792-plasmid-map-sequence-id-106481

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  34. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

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  35. pGS1522

    Plasmid
    #63794
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eVenus-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eVenus-Twin-Strep
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63794-plasmid-map-sequence-id-106482

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  36. pGS1422

    Plasmid
    #63793
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Twin-tag followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Twin-tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63793-plasmid-map-sequence-id-106479

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  37. pGS1512

    Plasmid
    #63788
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eVenus-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eVenus-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63788-plasmid-map-sequence-id-106476

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  38. pGS1312

    Plasmid
    #63786
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63786-plasmid-map-sequence-id-106475

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Showing: 8201 - 8240 of 9474 results