We narrowed to 24,612 results for: Nes

1. p5'UTRAct2 (GB0193)

   Plasmid

   #68178

   Purpose

   Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   5'UTR AtAct2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

   Plasmid

   #68213

   Purpose

   Transcriptional unit for Hygromycin resistance gene plant expression

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Hyg
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB1omega1_SF (GB0158)

   Plasmid

   #68242

   Purpose

   Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMyb12 (GB0075)

   Plasmid

   #68199

   Purpose

   Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Myb12 coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPAt2S3 (GB0029)

   Plasmid

   #68162

   Purpose

   Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   At2S3 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD_Mini35s(-60,UTR) (GB0050)

   Plasmid

   #68176

   Purpose

   Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   CaMV Mini35S promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Y344C

   Plasmid

   #24433

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C

   Promoter

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

8. L4C/S330C

   Plasmid

   #24436

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A,, L4C/S3…

   Promoter

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

9. K222C

   Plasmid

   #24438

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C

   Promoter

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

10. L4C

    Plasmid

    #24437

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, L4C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

11. S330C

    Plasmid

    #24439

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, "S…

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

12. K339CLM S330C

    Plasmid

    #24441

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, S330C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

13. K343CLM A337C

    Plasmid

    #24442

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, A337C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pcFNE3A (chick fibronectin E3A)

    Plasmid

    #14062

    Depositor

    Richard Hynes

    Article

    Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

    Insert

    Chicken Fibronectin EIIIA (FN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Blunted BstE2-AvaII fragment was inserted into bl…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pLXSN_HPV16E7∆DLYC

    Plasmid

    #136588

    Purpose

    Retroviral vector for expression of RB-binding mutant of HPVE7

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    HPV16E7∆DLYC
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    del aa 21-24, full length is 1-98

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS-u(L)Cas9

    Plasmid

    #127385

    Purpose

    Expresses Cas9 at low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(L)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-MKLP1

    Plasmid

    #70145

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111924

    Purpose

    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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