We narrowed to 4,899 results for: phy

1. pEAHISMRSA1376

   Plasmid

   #97002

   Purpose

   Expressing MRSA sar1376 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Putative 4-oxalocrotonate tautomerase
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEAHISMRSATAG

   Plasmid

   #97004

   Purpose

   Expressing MRSA TAG protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   DNA-3-methyladenine glycosylase I
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-10-3xFLAG-LGP2-MIIa

   Plasmid

   #58684

   Purpose

   Expresses LGP2 with a mutation to motif IIa in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

   Insert

   LGP2-MIIa (DHX58 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   mutation to Motif IIA, K138E and Y142F

   Promoter

   CMV

   Available since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

4. P2_DD-HNF4A8_C106R

   Plasmid

   #31096

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Flp/frt

   Tags

   FKBP L106P and myc

   Expression

   Mammalian

   Mutation

   splice variant 8 derived from promoter P2 with zi…

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHA TST 3C SMO EABR

   Plasmid

   #234989

   Purpose

   For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   SMO-EABR (SMO Human)
   

   Use

   Tags

   HA, Twin-Strep-Tag, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM2AH + Slo1

   Plasmid

   #16195

   Depositor

   Larry Salkoff

   Article

   McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

   Insert

   calcium-activated potassium channel (KCNMA1, Kcnma1 Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Ross Bathgate, Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   DCA (same as CAG promoter)

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid

    #208289

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA; mutations in iSpyMac

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Kif2c

    Plasmid

    #208651

    Purpose

    Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (KIF2C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-FRT-Rev-3xGFP

    Plasmid

    #191204

    Purpose

    FlpO dependent GFP expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    3X GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid

    #197509

    Purpose

    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Expression

    Mutation

    ABE8e mutations in TadA and nSpCas9(D10A)

    Promoter

    Cbh

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

    Plasmid

    #51509

    Purpose

    Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV1

    Insert

    tdTomato-T2A-SypEGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-FRT-Rev-TdTomato

    Plasmid

    #191203

    Purpose

    FlpO dependent TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HDM-SARS2-Spike-delta21

    Plasmid

    #155130

    Purpose

    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized to H. sapiens using IDT codon opt…

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-dnMCAK

    Plasmid

    #208650

    Purpose

    Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S92A , S106A , S108A, S112A , S186A, H530A, R534A…

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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