We narrowed to 28,391 results for: REP

1. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. phage UbiC NLS HA stdMCP stdHalo

   Plasmid

   #104999

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   stdMCP-stdHalo
   

   Use

   Lentiviral

   Tags

   HA tag, NLS, Two synonymous copies of the Halo-ta…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK mScarlet-LC3B

   Plasmid

   #200083

   Purpose

   Mammalian expression of fluorescently-tagged marker for autophagic vesicles

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   Map1lc3b (Map1lc3b Rat)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Human, Synthetic)
   

   Use

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

   Plasmid

   #174896

   Purpose

   Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-GSDMD-Myc

   Plasmid

   #201166

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

    Plasmid

    #85039

    Purpose

    For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    RFP-P2A-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MYR-FR-C2D-EGFR

    Plasmid

    #179262

    Purpose

    Light-induced clustering of EGFR cytosolic domains

    Depositor

    Jared Toettcher

    Article

    Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-phiC31-LP-TC

    Plasmid

    #183755

    Purpose

    AAVS1 targeting construct with the phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTet-O-NEUROD1-T2A-PuroR

    Plasmid

    #162338

    Purpose

    Lentiviral expression of NEUROD1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_Thy1StTA

    Plasmid

    #97411

    Purpose

    TET driver for amplified expression in cortical neurons

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse thy1PSs

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_hGH

    Plasmid

    #182410

    Purpose

    Bacterial expression of human growth hormone

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    hGH (GH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FLEX-SaCas9-U6-sgGrin1

    Plasmid

    #124852

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge