We narrowed to 20,225 results for: ACE;

1. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

   Plasmid

   #206080

   Purpose

   dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S25N

   Promoter

   CMV

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP Rab11a WT pcDNA3.1 Zeo (+)

   Plasmid

   #206079

   Purpose

   Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

   Plasmid

   #140014

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y126A, Y197F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK401

   Plasmid

   #110597

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS-Squ:mCherry

   Plasmid

   #20163

   Depositor

   Eric Wieschaus

   Article

   Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

   Insert

   spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pBEST-p70a-UTR3-deGFP-6xHis-T500

   Plasmid

   #92224

   Purpose

   Expression of deGFP using the p70a promoter and UTR3

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPKm-196

   Plasmid

   #90511

   Purpose

   pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PIF6-DBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET26b_Nb.AD102

   Plasmid

   #131403

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD102
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MTK8b_005

    Plasmid

    #123893

    Purpose

    Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' AAV ITR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK3b_021

    Plasmid

    #123802

    Purpose

    Encodes VPR activation domain as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VPR AD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK3a_026

    Plasmid

    #123778

    Purpose

    Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sadCAS9 (with NLS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-HisD-mNG-C-Kbp

    Plasmid

    #178013

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-C-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-HisD-mNG-Pa-Kbp

    Plasmid

    #178014

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Pa-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-HisD-mNG-Hv-Kbp

    Plasmid

    #178015

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Hv-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-HisD-mNG-D-Kbp

    Plasmid

    #178012

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-D-Kbp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Dec. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-RiboJ-mScarlet-T7

    Plasmid

    #173227

    Purpose

    Encodes mScarlet fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mScarlet
    

    Use

    Cell-free system

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. MTK0_014

    Plasmid

    #123929

    Purpose

    Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBIattB destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid

    #140201

    Purpose

    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Cloning of crrna an…

    Tags

    dLbCas12a crRNA scaffold

    Expression

    Mutation

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-synPCB2.0

    Plasmid

    #139477

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V413L

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV DCC mut

    Plasmid

    #16460

    Depositor

    Bert Vogelstein

    Article

    Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

    Insert

    DCC (DCC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' end of DCC

    Promoter

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MTK3_026

    Plasmid

    #123741

    Purpose

    Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Flamindo2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JPF0510

    Plasmid

    #124001

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NET30 pEGFP-N2 (815)

    Plasmid

    #62011

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET30 (MOSPD3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

26. MTK678_002

    Plasmid

    #123923

    Purpose

    Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BAC-AmpR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOP461

    Plasmid

    #85934

    Purpose

    Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 4G7 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPKm-118

    Plasmid

    #90491

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    pFR Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. E1b-GFP-Tol2-Gateway dre SE-zic2a P

    Plasmid

    #89385

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    zic2a (zic2a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SpRY dCas9 VPR

    Plasmid

    #188511

    Purpose

    Expresses FLAG tagged SpRY dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge