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We narrowed to 7,670 results for: 11

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Showing: 4121 - 4140 of 7670 results

  7. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαq-LgB97 (GNAQ Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134360-plasmid-map-sequence-id-306401

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  8. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

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  11. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

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  15. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  20. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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Showing: 4121 - 4140 of 7670 results