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pD649-HAsp-AMIGO2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156803

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

AMIGO2 (AMIGO2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClβ.CreON

Plasmid

#196996

Purpose

Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Article

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClβ -YFP

Use

AAV and Cre/Lox

Tags

YFP

Promoter

short CAG

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pOD_009

Plasmid

#159520

Purpose

Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-UbcM8C86A

Plasmid

#12441

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UbcM8 (Ube2l6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

C86A

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta GagPol

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR5A2_Hormone-recep

Plasmid

#109818

Purpose

Protein expression and purification of NR5A2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR5A2_Hormone-recep (NR5A2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35F5

Plasmid

#132085

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F5 (SLC35F5 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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YFP-Fibrillarin-C1

Plasmid

#134538

Purpose

Expresses YFP-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF5_P2A_Hygro_Barcode

Plasmid

#120491

Purpose

Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF5 (KLF5 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VPS4-E228Q (VPS4A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19 ACMV DNA-B 1.5mer

Plasmid

#159135

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-B 1.5mer

Use

Infectious clone for bombarding plants

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF785

Plasmid

#88459

Purpose

Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF785 (ZNF785 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35F3

Plasmid

#132109

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F3 (SLC35F3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35F4

Plasmid

#132097

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F4 (SLC35F4 Human)

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCI-neo:β2-365AAQA368 L349M

Plasmid

#35610

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 L349M 365AAQA368 (Chrnb2 mouse strain 129, Mouse)

Expression

Mammalian

Mutation

L349M and 365AAQA368

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1925

Plasmid

#105869

Purpose

High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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CHAF1B_pCSdest

Plasmid

#53862

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CHAF1B (CHAF1B Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSL1765 (pSPIN-R, R*MmeI)

Plasmid

#160734

Purpose

Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b His SUMO mNeon (loop1)PPM1H

Plasmid

#240002

Purpose

Expresses His SUMO mNeon (inserted in loop 1) PPM1H phosphatase in bacterial cells

Depositor

Suzanne Pfeffer

Article

Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

Insert

His SUMO mNeon (loop 1) PPM1H (PPM1H Human)

Expression

Bacterial

Available Since

Feb. 12, 2026

Availability

Academic Institutions and Nonprofits only

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