161,570 results

1. pcDNA_DENV2-NS2B3_V5

   Plasmid

   #115906

   Purpose

   Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

   Depositor

   Alan Rothman

   Article

   Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

   Insert

   DENV2 NS2B3 protease
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-sgRNA-CMV-GFP

   Plasmid

   #85451

   Purpose

   Express sgRNA in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pCMV-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCMV-EGFP

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX_EFS-Cre_ppt-del

   Plasmid

   #162073

   Purpose

   Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Insert

   Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EFS (part of insert)

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-MCS-MED1long-IDR-YFP-NLS

   Plasmid

   #145276

   Purpose

   Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MED1-IDR long (MED1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ilux pGEX(-)

   Plasmid

   #107879

   Purpose

   Bacterial expression of ilux for bioluminescence imaging

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

   Insert

   ilux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF06R

   Plasmid

   #42940

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Human L1 element (L1RE1 Human)
   

   Use

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV MIE, L1 5'UTR

   Available since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti X1 Puro empty (w610-1)

   Plasmid

   #20953

   Purpose

   3rd gen lentiviral empty control vector, Puro

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-FOXO3

   Plasmid

   #153142

   Purpose

   Expression of Flag tagged human FOXO3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXO3 (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4-p52

    Plasmid

    #23289

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBD2b pcDNA3.1

    Plasmid

    #78142

    Purpose

    Mammalian expression of MBD2b

    Depositor

    Heidi Schwarzenbach

    Article

    Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

    Insert

    MBD2b (MBD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid

    #123230

    Purpose

    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dyn1-pmCherryN1

    Plasmid

    #27697

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Dynamin 1 (Dnm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

15. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-puro

    Plasmid

    #45561

    Depositor

    Michael McVoy

    Article

    Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

    Insert

    Puromycin N-acetyl transferase
    

    Use

    Bifunctional selection protein

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Phcmv (Strong human cytomegalovirus immediate ear…

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIPZ-EGFP:LIMD1

    Plasmid

    #108230

    Purpose

    Lentiviral vector for Tet-inducible LIMD1 expression

    Depositor

    Kenneth Irvine

    Article

    Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIRV-NF-kB-eCFP

    Plasmid

    #118094

    Purpose

    Flourescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eCFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NF-kB responsive element

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas12Max-CBE

    Plasmid

    #195337

    Purpose

    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-CBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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