We narrowed to 29,515 results for: REP

1. 1203AC-CMV-Cas7.1-Y55A-mutant

   Plasmid

   #221542

   Purpose

   Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO5d.SFFV.Cas9.P2A.BSD

   Plasmid

   #181981

   Purpose

   Lentiviral Cas9 expression

   Depositor

   Jan-Henning Klusmann

   Insert

   Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV-mt4xi-GCaMP6f

   Plasmid

   #192075

   Purpose

   Fluorescent reporter for mitochondrial calcium

   Depositor

   Timothy Ryan

   Insert

   4 repeats of improved COX8 signal peptide plus GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pExp-TF

   Plasmid

   #112575

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-TriggerFactor and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKM154

   Plasmid

   #13036

   Depositor

   Kenan Murphy

   Article

   Murphy et al Gene. 2000 Apr 4. 246(1-2):321-30.

   Insert

   cat, sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pTre-tight-SV40-luciferase (496)

   Plasmid

   #65489

   Purpose

   Inducible expression of coding/noncoding genes

   Depositor

   Roderic Guigo, Rory Johnson

   Insert

   SV40-luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight Tre promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKK1 -pFlp-18::mito4x-GCaMP6f

   Plasmid

   #194969

   Purpose

   Fluorescent reporter of mitochondrial matrix Ca2+.

   Depositor

   Frederic Hoerndli

   Insert

   4 repeats of Cox8 presequence plus GCaMP6f
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Flp-18

   Available since

   June 21, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pPtGG-1

   Plasmid

   #89990

   Purpose

   pBR322-based entry vector for Gateway cloning

   Depositor

   Philip Weyman

   Article

   Bielinski et al PeerJ Preprints. 2017 5:e2907v1

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KBB610

   Plasmid

   #185111

   Purpose

   Pom152 full repeat domain in pEX-N-His for bacterial expression and purification

   Depositor

   Ken Belanger

   Insert

   POM152
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Pom152 Repeats-6His

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAIP4_5IHW

    Plasmid

    #234148

    Purpose

    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5IHW
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid

    #221543

    Purpose

    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7346

    Plasmid

    #217370

    Purpose

    sfGFP reporter for synthetic transcriptional variant of Pca rhtB ZTP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Pca rhtB synthetic transcriptional riboswitch sfGFP reporter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFPV25.1_dGFPmut3-LVA

    Plasmid

    #187378

    Purpose

    rpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_LVA
    

    Use

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    C-terminal LVA tail

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KBB611

    Plasmid

    #185112

    Purpose

    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification

    Depositor

    Ken Belanger

    Insert

    POM152
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Pom152 repeats 4&5-6His

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD148 ZF43x10-Spaced-Far_EYFP

    Plasmid

    #138894

    Purpose

    EYFP reporter vector for the ZF43x10-Spaced-Far promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x10-Spaced-Far
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x10-Spaced-Far

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD146 ZF43x6-Spaced-Far_EYFP

    Plasmid

    #138892

    Purpose

    EYFP reporter vector for the ZF43x6-Spaced-Far promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x6-Spaced-Far
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Spaced-Far

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD138 ZF43x10-Spaced-Mid_EYFP

    Plasmid

    #138888

    Purpose

    EYFP reporter vector for the ZF43x10-Spaced-Mid promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x10-Spaced-Mid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x10-Spaced-Mid

    Available since

    Jan. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD136 ZF43x6-Spaced-Mid_EYFP

    Plasmid

    #138886

    Purpose

    EYFP reporter vector for the ZF43x6-Spaced-Mid promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x6-Spaced-Mid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Spaced-Mid

    Available since

    Jan. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 371-R

    Plasmid

    #97033

    Purpose

    StrepII-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    StrepII tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSV40-mCherry-P2A-Hygro

    Plasmid

    #106478

    Purpose

    Expression of mCherry and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    mCherry
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pExp-Trx

    Plasmid

    #112577

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-TrxA and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pExp-MsyB

    Plasmid

    #112570

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pExp-mCherry

    Plasmid

    #112569

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pExp-Bla

    Plasmid

    #112561

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-Bla and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pExp-IF2

    Plasmid

    #112567

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-IF2 and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pExp-DsbC

    Plasmid

    #112564

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbC and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTre-Tight-luciferase-GFP (467)

    Plasmid

    #65491

    Purpose

    negative control for expression assays

    Depositor

    Roderic Guigo, Rory Johnson

    Insert

    EGFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLAX13 (CT#232)

    Plasmid

    #13918

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Use

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pCLIP18

    Plasmid

    #125142

    Purpose

    General purpose cloning vector

    Depositor

    Chris Boyd, David Sherratt

    Article

    Boyd et al Gene. 1995 Feb 3;153(1):57-62.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p2090 HPV-11 E2

    Plasmid

    #10874

    Depositor

    Peter Howley

    Article

    Del Vecchio et al J Virol. 1992 Oct . 66(10):5949-58.

    Insert

    HPV11 E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

41. N1-LOC8R

    Plasmid

    #66896

    Purpose

    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.

    Depositor

    Georg Ramm

    Insert

    LOC8R
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pExp-DsbA(SS)

    Plasmid

    #112563

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbA(SS) and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSLAX13 HA (CT#233)

    Plasmid

    #14027

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

44. pRL-TK 4x wt

    Plasmid

    #11313

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

    Insert

    4 bulged binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

45. pNC1053

    Plasmid

    #20406

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    linker: delta k

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNC829

    Plasmid

    #20386

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNC860

    Plasmid

    #20390

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Linker: ek

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. psiCHECK-2 GATD1 Alu_Mut1

    Plasmid

    #205470

    Purpose

    Luciferase reporter for Alu Domain Activity

    Depositor

    Glen Borchert

    Insert

    Mutant 1 GATD1 Alu Domain
    

    Use

    Luciferase and RNAi

    Tags

    Expression

    Mutation

    Mutated the entire structure of the alu domain wi…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSG7084 pG46-46

    Plasmid

    #232621

    Purpose

    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).

    Depositor

    Stephan Gruber

    Type

    Empty backbone

    Use

    Precursor plasmid to generate modified dna circles

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge